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Literature summary for 2.7.7.23 extracted from
- Purification and some properties of uridine diphosphate N-acetylglucosamine pyrophosphorylase from Neurospora crassa (1979), Can. J. Microbiol., 25, 1381-1386.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | slight stimulation | Neurospora crassa |  |
| dithioerythritol | highly stimulating | Neurospora crassa | |
| dithiothreitol | highly stimulating | Neurospora crassa | |
| glutathione | slight stimulation | Neurospora crassa | |
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol stabilizes, activity lost during storage can be recovered by addition of dithiothreitol | Neurospora crassa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
UDP-N-acetyl-D-glucosamine | pH 7.5, 30°C | Neurospora crassa | |
| 5.4 | - |
diphosphate | pH 7.5, 30°C | Neurospora crassa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially replace Mg2+ | Neurospora crassa | |
| Mg2+ | required for maximum activity | Neurospora crassa | |
| Mn2+ | can partially replace Mg2+ | Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-N-acetyl-D-glucosamine | Neurospora crassa | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | Neurospora crassa | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | Neurospora crassa IFO 6178 | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | Neurospora crassa IFO 6178 | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
| Neurospora crassa IFO 6178 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.66 | - |
purified enzyme, pH 7.5, 30°C | Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, phosphate buffer, pH 7.5, stable for at least 2 weeks | Neurospora crassa |
| 4°C, phosphate buffer, pH 7.5, 0.01 mM dithiothreitol, stable for at least 2 weeks | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-N-acetyl-D-glucosamine | highly specific, no activity with any other sugar nucloetide tested | Neurospora crassa | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Neurospora crassa | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | Neurospora crassa | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | highly specific, no activity with any other sugar nucloetide tested | Neurospora crassa IFO 6178 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Neurospora crassa IFO 6178 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin | Neurospora crassa IFO 6178 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
5 min, 50% of activity lost in the absence of dithiothreitol, 20% of activity lost in presence of dithiothreitol | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Neurospora crassa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Neurospora crassa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | most stable | Neurospora crassa |
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