Any feedback?
Literature summary for 2.7.7.23 extracted from
- Uridine diphosphoacetylglucosamine pyrophosphorylase (1959), J. Biol. Chem., 234, 1822-1827.
General Stability
| General Stability | Organism |
|---|---|
| labile toward fractionation with ammonium sulfate, inactivation can not be reversed by dialysis | Bos taurus |
| more stable toward ammonium sulfate then calf liver enzyme | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fluoride | inhibits Mg2+ but not Mn2+ activated enzyme | Bos taurus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for maximum activity | Staphylococcus aureus | |
| Mg2+ | required for maximum activity | Bos taurus | |
| Mn2+ | can partially replace Mg2+ | Staphylococcus aureus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-N-acetyl-D-glucosamine | Staphylococcus aureus | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | Bos taurus | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| organic solvents | labile to fractionation with | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Staphylococcus aureus |
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 330 | - |
purified enzyme, pH 7.4, 37°C | Bos taurus |
| 2390 | - |
purified enzyme, pH 7.4, 37°C | Staphylococcus aureus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, acetone precipitated enzyme, 3 months, no loss of activity | Bos taurus |
| -20°C, crude extract, 5-10% loss of activity per day of storage | Bos taurus |
| -20°C, purified enzyme or crude extract, 2 months, 10% loss of activity | Staphylococcus aureus |
| -20°C, purified enzyme, 10% loss of activity per week of storage | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-glucose | 30% of the activity with UDP-N-acetylglucosamine | Bos taurus | UTP + D-glucose 1-phosphate | - |
? | |
| diphosphate + UDP-N-acetyl-D-galactosamine | 2.8% of the activity with UDP-N-acetylglucosamine | Staphylococcus aureus | UTP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| diphosphate + UDP-N-acetyl-D-glucosamine | - |
Staphylococcus aureus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | - |
Bos taurus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Staphylococcus aureus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
| diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Bos taurus | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Staphylococcus aureus |
| 7 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | - |
Staphylococcus aureus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
