Cloned (Comment) | Organism |
---|---|
truncated and full length enzyme overexpressed in Escherichia coli, His-tag inserted for purification | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-130 | deletion of N-terminus, very low activity | Escherichia coli |
DELTA1-182 | deletion of N-terminus, very low activity | Escherichia coli |
DELTA1-227 | deletion of N-terminus, very low activity | Escherichia coli |
DELTA1-233 | deletion of N-terminus, very low activity | Escherichia coli |
DELTA1-250 | deletion of N-terminus | Escherichia coli |
DELTA1-26 | deletion of N-terminus | Escherichia coli |
DELTA1-78 | deletion of N-terminus, very low activity | Escherichia coli |
DELTA227-456 | deletion of C-terminus | Escherichia coli |
DELTA250-456 | deletion of C-terminus, low activity | Escherichia coli |
DELTA331-456 | deletion of C-terminus, roughly 50% of activity | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24700 | - |
SDS-PAGE, enzyme variant del233 | Escherichia coli |
25600 | - |
SDS-PAGE, enzyme variant del227 | Escherichia coli |
28600 | - |
SDS-PAGE, enzyme variant Tr250 | Escherichia coli |
36200 | - |
SDS-PAGE, enzyme variant del130 | Escherichia coli |
37100 | - |
SDS-PAGE, Tr331 | Escherichia coli |
41800 | - |
SDS-PAGE, enzyme variant del78 | Escherichia coli |
50100 | - |
SDS-PAGE, calculated from DNA sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | Escherichia coli | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | Escherichia coli JM83 | amino sugar metabolism | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
full length and truncated enzymes using His-tag | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Escherichia coli | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli JM83 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
diphosphate + UDP-N-acetyl-D-glucosamine | amino sugar metabolism | Escherichia coli JM83 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 50100, SDS-PAGE, gel filtration, all truncated forms form trimers except Tr250 (monomer) | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
More | bifunctional enzyme with activity of: glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
N-acetyl-D-glucosamine 1-phosphate | purified del227 enzyme, pH 7.4 | Escherichia coli | |
0.2 | - |
N-acetyl-D-glucosamine 1-phosphate | purified del233 enzyme, pH 7.4 | Escherichia coli | |
0.25 | - |
N-acetyl-D-glucosamine 1-phosphate | purified del78 enzyme, pH 7.4 | Escherichia coli | |
0.35 | - |
N-acetyl-D-glucosamine 1-phosphate | purified del130 enzyme, pH 7.4 | Escherichia coli | |
8 | - |
N-acetyl-D-glucosamine 1-phosphate | purified Tr250 enzyme, pH 7.4 | Escherichia coli | |
140 | - |
N-acetyl-D-glucosamine 1-phosphate | purified Tr331 enzyme, pH 7.4 | Escherichia coli | |
330 | - |
N-acetyl-D-glucosamine 1-phosphate | purified full length enzyme, pH 7.4 | Escherichia coli |