Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Corynebacterium ammoniagenes |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme E268D, hanging drop vapor diffusion method | Corynebacterium ammoniagenes |
Protein Variants | Comment | Organism |
---|---|---|
E268A | the mutant shows increased catalytic efficiency for FMN and reduced catalytic efficiency for ATP compared to the wild type enzyme | Corynebacterium ammoniagenes |
E268D | the mutant shows about wild type catalytic efficiencies for ATP and FMN | Corynebacterium ammoniagenes |
N210A | the mutant shows strongly reduced catalytic efficiencies for FMN and ATP compared to the wild type enzyme | Corynebacterium ammoniagenes |
N210D | the mutant shows strongly reduced catalytic efficiencies for FMN and ATP compared to the wild type enzyme | Corynebacterium ammoniagenes |
T208A | the mutant shows increased catalytic efficiency for FMN and reduced catalytic efficiency for ATP compared to the wild type enzyme | Corynebacterium ammoniagenes |
T208D | the mutant shows increased catalytic efficiency for FMN and increased catalytic efficiency for ATP compared to the wild type enzyme | Corynebacterium ammoniagenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00088 | - |
FMN | mutant enzyme E268A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.00088 | - |
FMN | mutant enzyme T208D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.00095 | - |
FMN | mutant enzyme T208A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.00117 | - |
FMN | mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.00119 | - |
FMN | wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.00823 | - |
FMN | mutant enzyme N210A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.01501 | - |
FMN | mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.03568 | - |
ATP | wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.0382 | - |
ATP | mutant enzyme T208D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.0387 | - |
ATP | mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.04537 | - |
ATP | mutant enzyme T208A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.04647 | - |
ATP | mutant enzyme E268A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.04704 | - |
ATP | mutant enzyme N210A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
0.07667 | - |
ATP | mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium ammoniagenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium ammoniagenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | - |
Corynebacterium ammoniagenes | diphosphate + FAD | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP:FMN adenylyltransferase | - |
Corynebacterium ammoniagenes |
FADS | - |
Corynebacterium ammoniagenes |
FMNAT | - |
Corynebacterium ammoniagenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
ATP | mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
4.3 | - |
ATP | mutant enzyme N210A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
7.3 | - |
ATP | mutant enzyme E268A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
7.7 | - |
ATP | mutant enzyme T208A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
8 | - |
ATP | wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
8.2 | - |
ATP | mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
8.5 | - |
ATP | mutant enzyme T208D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
19.7 | - |
FMN | mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
24.8 | - |
FMN | mutant enzyme N210A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
238.3 | - |
FMN | wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
270.2 | - |
FMN | mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
365.7 | - |
FMN | mutant enzyme T208D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
369.2 | - |
FMN | mutant enzyme T208A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
386 | - |
FMN | mutant enzyme E268A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes |