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Literature summary for 2.7.7.2 extracted from

  • Pedrolli, D.B.; Nakanishi, S.; Barile, M.; Mansurova, M.; Carmona, E.C.; Lux, A.; Gaertner, W.; Mack, M.
    The antibiotics roseoflavin and 8-demethyl-8-amino-riboflavin from Streptomyces davawensis are metabolized by human flavokinase and human FAD synthetase (2011), Biochem. Pharmacol., 82, 1853-1859.
    View publication on PubMed
Search for more literature for 2.7.7.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains BL21 and Rosetta (DE3) and in Pichia pastoris strain X33 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 FMN with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C Homo sapiens
0.109
-
 FMN without Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C Homo sapiens
0.116
-
 roseoflavin mononucleotide with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-nitrilotriacetic-agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + FMN
-
 Homo sapiens diphosphate + FAD
-
 ?
ATP + roseoflavin mononucleotide
-
 Homo sapiens diphosphate + roseoflavin adenine dinucleotide
-
 ?
additional information does not use 8-demethyl-8-amino-riboflavin mononucleotide as substrate Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 FMN without Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C Homo sapiens
0.07
-
 roseoflavin mononucleotide with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C Homo sapiens
0.08
-
 FMN with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.6
-
 roseoflavin mononucleotide with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C Homo sapiens
0.83
-
 FMN without Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C Homo sapiens
1.2
-
 FMN with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C Homo sapiens