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Literature summary for 2.7.7.2 extracted from

  • Brizio, C.; Galluccio, M.; Wait, R.; Torchetti, E.M.; Bafunno, V.; Accardi, R.; Gianazza, E.; Indiveri, C.; Barile, M.
    Over-expression in Escherichia coli and characterization of two recombinant isoforms of human FAD synthetase (2006), Biochem. Biophys. Res. Commun., 344, 1008-1016.
    View publication on PubMed
Search for more literature for 2.7.7.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain Rosetta(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
FMN 70% inhibited when using FMN concentrations higher than 0.004 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 FMN at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ strict requirement for MgCl2 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54190
-
 calculated from amino acid sequence Homo sapiens
60000
-
 SDS-PAGE, isoform 2 Homo sapiens
63000
-
 SDS-PAGE Homo sapiens
65270
-
 calculated from amino acid sequence Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NFF5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating Sepharose Fast Flow column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.59
-
 crude cell extract, at 37°C Homo sapiens
6.8
-
 after 12.5fold purification, at 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + FMN
-
 Homo sapiens diphosphate + FAD
-
 ?

Synonyms

Synonyms Comment Organism
FAD synthetase
-
 Homo sapiens
FADS1
-
 Homo sapiens
FADS2
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens