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Literature summary for 2.7.7.2 extracted from

  • Oka, M.; McCormick, D.B.
    Complete purification and general characterization of FAD synthetase from rat liver (1987), J. Biol. Chem., 262, 7418-7422.
    View publication on PubMed

General Stability

General Stability Organism
thiol maintains stability Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ 1 mM inhibits activity by 60% Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0094
-
FMN pH 7.5, 37°C Rattus norvegicus
0.053
-
ATP pH 7.5, 37°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 30% of the activity with Mg2+ Rattus norvegicus
Mg2+
-
Rattus norvegicus
Mn2+ about 15% of the activity with Mg2+ Rattus norvegicus
additional information Zn2+ cannot replace Mg2+ Rattus norvegicus
additional information Ba2+, Co2+, Cu2+, Cd2+, Fe2+, Ni2+, Sn2+ and Sr2+ do not show any activity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
2 * 53000, SDS-PAGE Rattus norvegicus
97000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + FMN Rattus norvegicus
-
diphosphate + FAD
-
ir

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
rat
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.133
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 1 mM dithiothreitol, 20% glycerol can be stored without significant loss of activity for 1 week Rattus norvegicus
5°C, if dithiothreitol is removed from the purified enzyme solution enzyme denatures within 12 h Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + FMN
-
Rattus norvegicus diphosphate + FAD
-
ir
ATP + FMN essentially irreversible in the direction of FAD formation Rattus norvegicus diphosphate + FAD
-
ir

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
enzyme denatured as temperature increases, completely inactivated above, bovine serum albumin stabilizes up to 45°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
-
Rattus norvegicus