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Literature summary for 2.7.7.19 extracted from

  • Lapkouski, M.; Haellberg, B.M.
    Structure of mitochondrial poly(A) RNA polymerase reveals the structural basis for dimerization, ATP selectivity and the SPAX4 disease phenotype (2015), Nucleic Acids Res., 43, 9065-9075.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
mature enzyme, to 1.82 A resolution. Enzyme crystallizes as a dimer and consists of a N-terminal domain (NTD: 52-194 aa) and of palm (195-341 aa) and fingers (342-527 aa). The palm domain harbors the catalytic triad, residue D237, located at the base of sheet beta6, D239 on strand beta6 and D319, which belongs to beta10. Structures of mtPAP in complex with cosubstrates UTP, CTP and GTP indicate that initial nucleotide selection occurs in the absence of a template Gallus gallus

Protein Variants

Protein Variants Comment Organism
D237N loss of activity. A heterodimer in which one monomer is active and the other is not retains activity Gallus gallus
K112E disruption of a a continuous positively charged surface formed upon dimerization, decrease in activity Gallus gallus
K76E/K80E/K81E disruption of a a continuous positively charged surface formed upon dimerization, decrease in activity Gallus gallus
R272E complete loss of activity Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Gallus gallus 5739
-

Organism

Organism UniProt Comment Textmining
Gallus gallus F1NBW0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA primer
-
Gallus gallus diphosphate + RNA primer-A
-
?
CTP + RNA primer enzyme can use UTP, CTP and GTP as co-substrates in vitro Gallus gallus diphosphate + RNA primer-C enzyme can use UTP, CTP and GTP as co-substrates in vitro ?
GTP + RNA primer
-
Gallus gallus diphosphate + RNA primer-G enzyme can use UTP, CTP and GTP as co-substrates in vitro ?
UTP + RNA primer
-
Gallus gallus diphosphate + RNA primer-U enzyme can use UTP, CTP and GTP as co-substrates in vitro ?

Synonyms

Synonyms Comment Organism
MTPAP
-
Gallus gallus