Literature summary for 2.7.7.19 extracted from

Balbo, P.B.; Meinke, G.; Bohm, A.
Kinetic studies of yeast polyA polymerase indicate an induced fit mechanism for nucleotide specificity (2005), Biochemistry, 44, 7777-7786.

Search for more literature for 2.7.7.19

Protein Variants

Protein Variants	Comment	Organism
additional information	mutation of bovine residue N202 (equivalent to yeast N189) to alanine has essentially no effect on the apparent Km for ATP, but has a pronounced effect on the apparent Vmax, suggesting that this residue is particularly important in the recognition of the adenine of ATP	Saccharomyces cerevisiae

General Stability

General Stability	Organism
a decay of PAP activity with a half-life of approximately 2 h (on ice) is observed when Nonidet-P 40 is omitted from the enzyme storage solution	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
alpha, beta-methylene-ATP	a nonreactive ATP analogue	Saccharomyces cerevisiae
diphosphate	product inhibitor	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0263	-	oligo(A)17C	incubation at 30 °C for 15 min in the presence of MgATP2-	Saccharomyces cerevisiae
0.0359	-	ATP	-	Saccharomyces cerevisiae
0.0468	-	oligo(A)18	incubation at 30 °C for 15 min in the presence of MgATP2-	Saccharomyces cerevisiae
0.0642	-	oligo(A)18	incubation at 30 °C for 15 min in the presence of MgCTP2-	Saccharomyces cerevisiae
0.1036	-	CTP	-	Saccharomyces cerevisiae
0.123	-	ATP	incubation at 30 °C for 15 min, using oligo(A)17C (a modification of the 3'-terminal residue from A to C)	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant yeast PAP is purified by nickel affinity and anion exchange chromatography using NTA agarose and Source S resins	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + oligo(A)17C	-	Saccharomyces cerevisiae	diphosphate + oligo(A)18C	-	?
ATP + oligo(A)18	-	Saccharomyces cerevisiae	diphosphate + oligo(A)19	-	?
ATP + RNAn	-	Saccharomyces cerevisiae	diphosphate + RNAn+1	-	?
CTP + RNAn	-	Saccharomyces cerevisiae	diphosphate + RNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
PAP	-	Saccharomyces cerevisiae
polyA polymerase	-	Saccharomyces cerevisiae
polyadenylate polymerase	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)