Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.19 extracted from

  • Raynal, L.C.; Carpousis, A.J.
    Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation (1999), Mol. Microbiol., 32, 765-775.
    View publication on PubMed
Search for more literature for 2.7.7.19

Protein Variants

Protein Variants Comment Organism
D170A diphosphate release equivalent to AMP production, 5-30% of wild type activity Escherichia coli
D170P diphosphate release equivalent to AMP production, 5-30% of wild type activity Escherichia coli
D170P no detectable AMP incorporation Escherichia coli
D214A diphosphate release equivalent to AMP production, 5-30% of wild type activity Escherichia coli
D214P diphosphate release equivalent to AMP production, 5-30% of wild type activity Escherichia coli
D214P no detectable AMP incorporation Escherichia coli
D79A disparity between Diphosphate release and AMP incorporation Escherichia coli
D88A diphosphate release equivalent to AMP production, 5-30% of wild type activity Escherichia coli
D88P diphosphate release equivalent to AMP production, 5-30% of wild type activity Escherichia coli
D88P no detectable AMP incorporation Escherichia coli
D90A diphosphate release equivalent to AMP production, 5-30% of wild type activity Escherichia coli
D90P diphosphate release equivalent to AMP production, 5-30% of wild type activity Escherichia coli
D90P no detectable AMP incorporation Escherichia coli
G74A disparity between Diphosphate release and AMP incorporation Escherichia coli
G74P disparity between Diphosphate release and AMP incorporation Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
NaF 10 mM, complete inhibition Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ more active in presence of Mn2+ than Mg2+ Escherichia coli
Mn2+ more active in presence of Mn2+ than Mg2+ Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-