Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | dependence on divalent cation concentration | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal concentration: 60 mM | Saccharomyces cerevisiae | |
Mg2+ | more active in presence of Mn2+ than Mg2+ | Saccharomyces cerevisiae | |
Mg2+ | optimal concentration depends on ATP concentration | Saccharomyces cerevisiae | |
Mn2+ | more active in presence of Mn2+ than Mg2+ | Saccharomyces cerevisiae | |
Mn2+ | optimal concentration depends on ATP concentration | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
gel filtration | Saccharomyces cerevisiae |
63000 | - |
1 * 63000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | highly specific for ATP | Saccharomyces cerevisiae | diphosphate + RNA(A)n | no apparent length limitation for the poly(A) tail synthesized | ? | |
ATP + RNA | primer: various E. coli tRNAs or rRNAs | Saccharomyces cerevisiae | diphosphate + RNA(A)n | no apparent length limitation for the poly(A) tail synthesized | ? | |
ATP + RNA | elongation of the primer is distributive | Saccharomyces cerevisiae | diphosphate + RNA(A)n | no apparent length limitation for the poly(A) tail synthesized | ? | |
ATP + RNA | primer required | Saccharomyces cerevisiae | diphosphate + RNA(A)n | no apparent length limitation for the poly(A) tail synthesized | ? | |
ATP + RNA | primer: RNA homopolymers | Saccharomyces cerevisiae | diphosphate + RNA(A)n | no apparent length limitation for the poly(A) tail synthesized | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
Mn2+-activated enzyme | Saccharomyces cerevisiae |