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Literature summary for 2.7.7.19 extracted from

  • Sippel, A.E.
    Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli (1973), Eur. J. Biochem., 37, 31-40.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3',5'-AMP slight stimulation Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
adenylyl-(3'-5')adenosine
-
Escherichia coli
Adenylyl-(3'-5')cytosine
-
Escherichia coli
aurintricarboxylic acid
-
Escherichia coli
Calf thymus DNA
-
Escherichia coli
diphosphate
-
Escherichia coli
phosphate
-
Escherichia coli
poly(C)
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ more active in presence of Mn2+ than Mg2+ Escherichia coli
Mg2+ in presence of Mg2+ and a specificity factor required for correct cleavage at the poly(A) site of pre-mRNA Escherichia coli
Mn2+ more active in presence of Mn2+ than Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
1 * 50000, SDS-PAGE Escherichia coli
58000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli
high salt conditions required during purification Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA highly specific for ATP Escherichia coli diphosphate + RNA(A)n
-
?
ATP + RNA primer required Escherichia coli diphosphate + RNA(A)n
-
?
ATP + RNA enzyme is unable to catalyze pyrophosphorolysis or phosphorolysis reaction Escherichia coli diphosphate + RNA(A)n
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 pH 7.5: 50% of maximum activity, pH 9.0: 15% of maximum activity Escherichia coli