Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3',5'-AMP | slight stimulation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenylyl-(3'-5')adenosine | - |
Escherichia coli | |
Adenylyl-(3'-5')cytosine | - |
Escherichia coli | |
aurintricarboxylic acid | - |
Escherichia coli | |
Calf thymus DNA | - |
Escherichia coli | |
diphosphate | - |
Escherichia coli | |
phosphate | - |
Escherichia coli | |
poly(C) | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | more active in presence of Mn2+ than Mg2+ | Escherichia coli | |
Mg2+ | in presence of Mg2+ and a specificity factor required for correct cleavage at the poly(A) site of pre-mRNA | Escherichia coli | |
Mn2+ | more active in presence of Mn2+ than Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000, SDS-PAGE | Escherichia coli |
58000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
high salt conditions required during purification | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | highly specific for ATP | Escherichia coli | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | primer required | Escherichia coli | diphosphate + RNA(A)n | - |
? | |
ATP + RNA | enzyme is unable to catalyze pyrophosphorolysis or phosphorolysis reaction | Escherichia coli | diphosphate + RNA(A)n | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | pH 7.5: 50% of maximum activity, pH 9.0: 15% of maximum activity | Escherichia coli |