Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Rattus norvegicus | |
additional information | not inhibitory: alpha-amanitin | Rattus norvegicus | |
NH4+ | ammonium sulfate; maximal activity at 33 mM, inhibition above 150 mM | Rattus norvegicus | |
rifamycin AF/013 | O-n-octyloxime of 3-formylrifamycin SV | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
ATP | 37°C, pH 8.0 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | maximal activity at 33 mM, inhibition above 150 mM | Rattus norvegicus | |
Mg2+ | more active in presence of Mn2+ than Mg2+ | Rattus norvegicus | |
Mn2+ | required | Rattus norvegicus | |
Mn2+ | more active in presence of Mn2+ than Mg2+ | Rattus norvegicus | |
Mn2+ | optimal concentration: 0.25-1.0 mM | Rattus norvegicus | |
NH4+ | maximal activity at 33 mM, inhibition above 150 mM | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
mitochondria, glycerol density gradient centrifugation | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatoma cell | Morris hepatoma tumor cells 3924A | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-90°C, 50% glycerol, stable for 4 weeks, about 40% loss of activity after 5 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA | highly specific for ATP | Rattus norvegicus | diphosphate + RNA(A)n | average length of poly(A) formed is 600 nucleotides | ? | |
ATP + RNA | primer required | Rattus norvegicus | diphosphate + RNA(A)n | average length of poly(A) formed is 600 nucleotides | ? | |
ATP + RNA | poly(A) is the most effective primer | Rattus norvegicus | diphosphate + RNA(A)n | average length of poly(A) formed is 600 nucleotides | ? | |
ATP + RNA | mitochondrial RNA at least five times more efficiently used than nuclear RNA | Rattus norvegicus | diphosphate + RNA(A)n | average length of poly(A) formed is 600 nucleotides | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | - |
Rattus norvegicus |