Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21 with a His-tag | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
crystals of the apo-enzyme belong to space group: P21 with 58.5% solvent and contain four molecules of NaMN AT in the asymmetric subunit, crystals in complex with deamido-NAD+ belong to space group: P212121 with 55.6% solvent and contain six molecules of NaMN AT in the asymmetric subunit, conditions: polyethylene glycol 3350 and 100 mM MgCl2 | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NMN | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
deamido-NAD+ | 37°C, pH 7.4 | Bacillus subtilis | |
0.043 | - |
nicotinamide ribonucleotide | 37°C, pH 7.4 | Bacillus subtilis | |
0.135 | - |
ATP | 37°C, pH 7.4 | Bacillus subtilis | |
1.7 | - |
NAD+ | 37°C, pH 7.4 | Bacillus subtilis | |
2.3 | - |
NMN | 37°C, pH 7.4 | Bacillus subtilis | |
4.2 | - |
diphosphate | 37°C, pH 7.4 | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36370 | 49140 | gel filtration, sedimentation equlibrium centrifugation | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.054 | - |
NMN as substrate | Bacillus subtilis |
0.34 | - |
NAD+ as substrate | Bacillus subtilis |
2.8 | - |
deamido-NAD+ as substrate | Bacillus subtilis |
10.1 | - |
ATP as substrate | Bacillus subtilis |
11.1 | - |
nicotinate ribonucleotide as substrate | Bacillus subtilis |
11.5 | - |
diphosphate as substrate | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Bacillus subtilis | diphosphate + deamido-NAD+ | - |
r | |
ATP + NMN | - |
Bacillus subtilis | diphosphate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallization studies | Bacillus subtilis |