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Literature summary for 2.7.7.15 extracted from

  • Brault, J.P.; Friesen, J.A.
    Characterization of cytidylyltransferase enzyme activity through high performance liquid chromatography (2016), Anal. Biochem., 510, 26-32.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the full length CCT requires activation via association with lipids and has been reported to have activity 50fold lower in the absence of lipids when compared to the activity of CCTalpha236 Rattus norvegicus

Application

Application Comment Organism
analysis CTP:phosphocholine cytidylyltransferase enzyme assay that employs separation of non-radioactive CDP-choline from CTP. The assay was tested using a truncated version of rat CCTalpha by measuring CDP-choline product formation, and the HPLC method can be applied to glycerol 3-phosphate cytidylyltransferase and CTP:2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase synthetase (CMS), members of the cytidylyltransferase family that produce CDP-glycerol and CDP-methylerythritol, respectively Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information construction of a truncated version of rat CCTalpha, CCTalpha236. The full length CCT requires activation via association with lipids and has been reported to have activity 50fold lower in the absence of lipids when compared to the activity of CCTalpha236 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.49
-
phosphocholine K0.5 value, Hill coefficient 0.992, pH 7.0, 37°C Rattus norvegicus
2.49
-
phosphocholine pH 7.0, 37°C, enzyme mutant CCTalpha236 Rattus norvegicus
3.15
-
CTP pH 7.0, 37°C, enzyme mutant CCTalpha236 Rattus norvegicus
4.07
-
CTP K0.5 value, Hill coefficient 1.59, pH 7.0, 37°C Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + phosphocholine Rattus norvegicus
-
diphosphate + CDP-choline
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant truncated enzyme mutant CCTalpha236 by CM-sepharose chromatography Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.85
-
truncated enzyme mutant CCTalpha236, pH 7.0,37°C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + phosphocholine
-
Rattus norvegicus diphosphate + CDP-choline
-
?
additional information development of an assay method for determination of the catalytic activity measuring the production of radiolabeled CDP-choline from 14C-labeled phosphocholine, and separation of non-radioactive CDP-choline from CTP by C18 reverse phase HPLC using a mobile phase of 0.1 M ammonium bicarbonate (98%) and acetonitrile (2%) at pH 7.4, evaluation, overview Rattus norvegicus ?
-
?

Synonyms

Synonyms Comment Organism
CCT
-
Rattus norvegicus
CCTalpha236
-
Rattus norvegicus
choline phosphate cytidylyltransferase
-
Rattus norvegicus
CTP:phosphocholine cytidylyltransferase
-
Rattus norvegicus
cytidylyltransferase
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Rattus norvegicus

General Information

General Information Comment Organism
evolution the enzyme is a member of the cytidylyltransferase family of enzymes that utilize cytidine 5'-triphosphate (CTP) to synthesize molecules that are typically precursors to membrane phospholipids Rattus norvegicus
metabolism the enzyme catalyzes conversion of phosphocholine and CTP to cytidine diphosphocholine (CDP-choline), a step critical for synthesis of the membrane phospholipid phosphatidylcholine Rattus norvegicus