Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the full length CCT requires activation via association with lipids and has been reported to have activity 50fold lower in the absence of lipids when compared to the activity of CCTalpha236 | Rattus norvegicus |
Application | Comment | Organism |
---|---|---|
analysis | CTP:phosphocholine cytidylyltransferase enzyme assay that employs separation of non-radioactive CDP-choline from CTP. The assay was tested using a truncated version of rat CCTalpha by measuring CDP-choline product formation, and the HPLC method can be applied to glycerol 3-phosphate cytidylyltransferase and CTP:2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase synthetase (CMS), members of the cytidylyltransferase family that produce CDP-glycerol and CDP-methylerythritol, respectively | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a truncated version of rat CCTalpha, CCTalpha236. The full length CCT requires activation via association with lipids and has been reported to have activity 50fold lower in the absence of lipids when compared to the activity of CCTalpha236 | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.49 | - |
phosphocholine | K0.5 value, Hill coefficient 0.992, pH 7.0, 37°C | Rattus norvegicus | |
2.49 | - |
phosphocholine | pH 7.0, 37°C, enzyme mutant CCTalpha236 | Rattus norvegicus | |
3.15 | - |
CTP | pH 7.0, 37°C, enzyme mutant CCTalpha236 | Rattus norvegicus | |
4.07 | - |
CTP | K0.5 value, Hill coefficient 1.59, pH 7.0, 37°C | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphocholine | Rattus norvegicus | - |
diphosphate + CDP-choline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant truncated enzyme mutant CCTalpha236 by CM-sepharose chromatography | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.85 | - |
truncated enzyme mutant CCTalpha236, pH 7.0,37°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphocholine | - |
Rattus norvegicus | diphosphate + CDP-choline | - |
? | |
additional information | development of an assay method for determination of the catalytic activity measuring the production of radiolabeled CDP-choline from 14C-labeled phosphocholine, and separation of non-radioactive CDP-choline from CTP by C18 reverse phase HPLC using a mobile phase of 0.1 M ammonium bicarbonate (98%) and acetonitrile (2%) at pH 7.4, evaluation, overview | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCT | - |
Rattus norvegicus |
CCTalpha236 | - |
Rattus norvegicus |
choline phosphate cytidylyltransferase | - |
Rattus norvegicus |
CTP:phosphocholine cytidylyltransferase | - |
Rattus norvegicus |
cytidylyltransferase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the cytidylyltransferase family of enzymes that utilize cytidine 5'-triphosphate (CTP) to synthesize molecules that are typically precursors to membrane phospholipids | Rattus norvegicus |
metabolism | the enzyme catalyzes conversion of phosphocholine and CTP to cytidine diphosphocholine (CDP-choline), a step critical for synthesis of the membrane phospholipid phosphatidylcholine | Rattus norvegicus |