Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Lipids | regulation of activity by reversible association with membranes containing anionic lipids | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
native and mutant enzyme expressed as His-tag fusion proteins in COS-1 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C113S | no significant effect of activity | Rattus norvegicus |
C139S | no significant effect of activity | Rattus norvegicus |
C354S | no significant effect of activity | Rattus norvegicus |
C359S | no significant effect of activity | Rattus norvegicus |
C37S | unable to form dimers | Rattus norvegicus |
C68S | no significant effect of activity | Rattus norvegicus |
C73S | no significant effect of activity | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | enzyme is activated when bound to vesicles | Rattus norvegicus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Rattus norvegicus |
80000 | 110000 | diffuse band in SDS-PAGE using chemically cross-linked protein | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + choline phosphate | Rattus norvegicus | rate limiting enzyme for the synthesis of phosphatidylcholine | diphosphate + CDP-choline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes using His-tag | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + choline phosphate | - |
Rattus norvegicus | diphosphate + CDP-choline | - |
? | |
CTP + choline phosphate | rate limiting enzyme for the synthesis of phosphatidylcholine | Rattus norvegicus | diphosphate + CDP-choline | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
CCTalpha | - |
Rattus norvegicus |