Application | Comment | Organism |
---|---|---|
drug development | given the absence of PE synthesis in red blood cells, PfECT represents a potential antimalarial target opening the way for a rational conception of bioactive compounds | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
PfECT, sequence comparison, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
H146A | site-directed mutagenesis, the mutant is almost inactive | Plasmodium falciparum |
H422A | site-directed mutagenesis, the mutant shows increased kcat and Vmax with ethanolamine phosphate compared to the wild-type enzyme | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics of wild-type and mutant enzymes, overview | Plasmodium falciparum | |
0.373 | - |
Ethanolamine phosphate | pH 8.0, 37°C, native enzyme | Plasmodium falciparum | |
0.374 | - |
CTP | pH 8.0, 37°C, recombinant His-tagged mutant H146A enzyme | Plasmodium falciparum | |
0.452 | - |
Ethanolamine phosphate | pH 8.0, 37°C, recombinant His-tagged wild-type enzyme | Plasmodium falciparum | |
0.465 | - |
CTP | pH 8.0, 37°C, recombinant His-tagged mutant H422A enzyme | Plasmodium falciparum | |
0.565 | - |
Ethanolamine phosphate | pH 8.0, 37°C, recombinant His-tagged mutant H422A enzyme | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | cellular localization and expression of PfECT along the parasite life cycle, overview | Plasmodium falciparum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + ethanolamine phosphate | Plasmodium falciparum | - |
diphosphate + CDP-ethanolamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8IDM2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + ethanolamine phosphate | - |
Plasmodium falciparum | diphosphate + CDP-ethanolamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | homology modelling, three-dimensional structural model of Pf ECT, overview | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
CTP:phosphoethanolamine CT | - |
Plasmodium falciparum |
CTP:phosphoethanolamine cytidylyltransferase | - |
Plasmodium falciparum |
ECT | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
Ethanolamine phosphate | pH 8.0, 37°C, recombinant His-tagged wild-type enzyme | Plasmodium falciparum | |
4.1 | - |
Ethanolamine phosphate | pH 8.0, 37°C, recombinant His-tagged mutant H422A enzyme | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
malfunction | inhibition of PE biosynthesis leads to parasite death | Plasmodium falciparum |
metabolism | the enzyme catalyzes the rate-limiting step of the PE metabolic pathway in the parasite | Plasmodium falciparum |
additional information | the N-terminal CT domain is the only catalytically active domain of the enzyme. The inactive C-terminal domain is important for dimer stabilization. Homology modelling, three-dimensional structural model of Pf ECT, overview | Plasmodium falciparum |
physiological function | phosphatidylethanolamine is mainly synthesized de novo by the CDP:ethanolamine-dependent Kennedy pathway. Plasmodium falciparum requires massive synthesis of phosphatidylethanolamine that together with phosphatidylcholine constitute the bulk of the malaria membrane lipids | Plasmodium falciparum |