Cloned (Comment) | Organism |
---|---|
gene OsVTC1-1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Oryza sativa Japonica Group |
gene OsVTC1-3, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Oryza sativa Japonica Group |
gene OsVTC1-8, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Oryza sativa Japonica Group |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of OsVTC1-1 silenced mutant plants by RNAi method | Oryza sativa Japonica Group |
additional information | generation of OsVTC1-3 silenced mutant plants by RNAi method | Oryza sativa Japonica Group |
additional information | generation of OsVTC1-8 silenced mutant plants by RNAi method | Oryza sativa Japonica Group |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Oryza sativa Japonica Group |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Oryza sativa Japonica Group | - |
diphosphate + GDP-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q6Z9A3 | OsVTC1-8 or GMPP3 | - |
Oryza sativa Japonica Group | Q84JH5 | - |
- |
Oryza sativa Japonica Group | Q84JH5 | OsVTC1-3 or GMPP1 | - |
Oryza sativa Japonica Group | Q941T9 | - |
- |
Oryza sativa Japonica Group | Q941T9 | OsVTC1-1 or GMPP2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Oryza sativa Japonica Group |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa Japonica Group | - |
leaf | expression is high in leaf | Oryza sativa Japonica Group | - |
leaf | high light-induced expression level | Oryza sativa Japonica Group | - |
additional information | OsVTC1-8 transcript level is low in both leaves and roots | Oryza sativa Japonica Group | - |
root | - |
Oryza sativa Japonica Group | - |
root | expression is high in root | Oryza sativa Japonica Group | - |
root | high dark-induced expression level | Oryza sativa Japonica Group | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | - |
Oryza sativa Japonica Group | diphosphate + GDP-mannose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDP-D-mannose pyrophosphorylase | - |
Oryza sativa Japonica Group |
GDP-mannose pyrophosphorylase | - |
Oryza sativa Japonica Group |
GMPase | - |
Oryza sativa Japonica Group |
GMPP1 | - |
Oryza sativa Japonica Group |
GMPP2 | - |
Oryza sativa Japonica Group |
GMPP3 | - |
Oryza sativa Japonica Group |
Os01g0847200 | - |
Oryza sativa Japonica Group |
Os03g0268400 | - |
Oryza sativa Japonica Group |
OsVTC1-1 | - |
Oryza sativa Japonica Group |
OsVTC1-3 | - |
Oryza sativa Japonica Group |
OsVTC1-8 | - |
Oryza sativa Japonica Group |
VCT1-1 | - |
Oryza sativa Japonica Group |
VCT1-3 | - |
Oryza sativa Japonica Group |
VTC1-1 | - |
Oryza sativa Japonica Group |
VTC1-3 | - |
Oryza sativa Japonica Group |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryza sativa Japonica Group |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Oryza sativa Japonica Group |
Organism | Comment | Expression |
---|---|---|
Oryza sativa Japonica Group | expression is inhibited by dark | down |
Oryza sativa Japonica Group | the expression of OsVTC1-1 is inhibited in roots in the dark | down |
Oryza sativa Japonica Group | the expression of OsVTC1-3 is inhibited in roots in the light | down |
Oryza sativa Japonica Group | expression is induced by light | up |
Oryza sativa Japonica Group | expression is induced by the dark | up |
Oryza sativa Japonica Group | expression is induced by dark | up |
Oryza sativa Japonica Group | the expression of OsVTC1-1 is induced in roots in the light | up |
Oryza sativa Japonica Group | the expression of OsVTC1-3 is induced in roots in the dark | up |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of OsVTC1-8 does not restore the ascorbic acid synthesis of the vtc1-1 mutant in Arabidopsis | Oryza sativa Japonica Group |
metabolism | enzyme OsVTC1-1 and OsVTC1-3 have higher GMPase activities than enzyme OsVTC1-8 in vitro. Only OsVTC1-1 and OsVTC1-3 are involved in ascorbic acid synthesis in rice | Oryza sativa Japonica Group |
physiological function | in VTC1-1 RNAi plants, the ascorbic acid content of rice leaves decreases, and the ascorbic acid production induced by light is limited. The overexpression of isoform VTC1-1 or OsVTC1-3 restores the ascorbic acid synthesis of a vtc1-1 mutant in Arabidopsis thaliana | Oryza sativa Japonica Group |
physiological function | VTC1-3 RNAi lines alter ascorbic acid synthesis levels in rice roots, but not in the leaves or under the light/dark treatment. The overexpression of isooform VTC1-1 or OsVTC1-3 restores the ascorbic acid synthesis of a vtc1-1 mutant in Arabidopsis thaliana | Oryza sativa Japonica Group |
physiological function | enzyme OsVTC1-1 may be involved in the ascorbic acid synthesis, which takes place in leaves. Overexpression of OsVTC1-1 restores the ascorbic acid synthesis of the vtc1-1 mutant in Arabidopsis | Oryza sativa Japonica Group |
physiological function | enzyme OsVTC1-3 may be responsible for ascorbic acid synthesis in roots. Overexpression of OsVTC1-3 restores the ascorbic acid synthesis of the vtc1-1 mutant in Arabidopsis | Oryza sativa Japonica Group |
physiological function | the overexpression of VTC1-1 restores the ascorbic acid synthesis of a VTC1-1 mutant in Arabidopsis. In VTC1-1 RNAi plants, the ascorbic acid content of rice leaves decreases, and the ascorbic acid production induced by light is limited | Oryza sativa Japonica Group |
physiological function | the overexpression of VTC1-3 restores the ascorbic acid synthesis of a VTC1-1 mutant in Arabidopsis. Isoform VTC1-3 RNAi lines alter ascorbic acid synthesis levels in rice roots, but not in the leaves or under the light/dark treatment | Oryza sativa Japonica Group |