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Literature summary for 2.7.7.13 extracted from

  • Hashimoto, H.; Sakakibara, A.; Yamasaki, M.; Yoda, K.
    Saccharomyces cerevisiae VIG9 encodes GDP-mannose pyrophosphorylase, which is essential for protein glycosylation (1997), J. Biol. Chem., 272, 16308-16314.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-fusion protein in Escherichia coli Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39565
-
x * 39565, deduced from nucleotide sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate Saccharomyces cerevisiae
-
GDPmannose + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P41940 GDP-mannose pyrophosphorylase is encoded by VIG9
-

Purification (Commentary)

Purification (Comment) Organism
GST-fusion protein, glutathione-Sepharose 4B beads Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate
-
Saccharomyces cerevisiae GDPmannose + diphosphate
-
r

Subunits

Subunits Comment Organism
? x * 39565, deduced from nucleotide sequence Saccharomyces cerevisiae