Literature summary for 2.7.7.12 extracted from

Crews, C.; Wilkinson, K.D.; Wells, L.; Perkins, C.; Fridovich-Keil, J.L.
Functional consequence of substitutions at residue 171 in human galactose-1-phosphate uridylyltransferase (2000), J. Biol. Chem., 275, 22847-22853.

Search for more literature for 2.7.7.12

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutants in Saccharomyces cerevisiae	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
F171L	site-directed mutagenesis, 10fold decreased activity	Homo sapiens
F171S	site-directed mutagenesis, no activity	Homo sapiens
F171W	site-directed mutagenesis, severely reduced abundance	Homo sapiens
F171Y	site-directed mutagenesis, 4% activity compared to wild-type, no inhibition by excess UDP-glucose	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
UDP-glucose	at high concentrations, wild-type and mutants F171L and F171S, not F171Y	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	native mutant F171S	-

Purification (Commentary)

Purification (Comment)	Organism
His-tagged recombinant wild-type and mutants F171Y and F171L to near homogeneity	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + alpha-D-galactose 1-phosphate	-	Homo sapiens	alpha-D-glucose 1-phosphate + UDP-galactose	-	r

Subunits

Subunits	Comment	Organism
More	modeling of the human enzyme structure onto the crystal structure of the Escherichia coli enzyme	Homo sapiens

Synonyms

Synonyms	Comment	Organism
GALT	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.6	-	alpha-D-galactose 1-phosphate	recombinant wild-type, pH 8.7	Homo sapiens
9.3	-	alpha-D-galactose 1-phosphate	recombinant wild-type, pH 8.7	Homo sapiens
98	-	alpha-D-galactose 1-phosphate	recombinant wild-type, pH 8.7	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.7	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)