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Literature summary for 2.7.7.12 extracted from
- Human galactose-1-phosphate uridylyltransferase: purification and comparison of the red blood cell and placental enzymes (1982), Arch. Biochem. Biophys., 216, 503-511.
General Stability
| General Stability | Organism |
|---|---|
| UDP-glucose stabilizes during gel filtration | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
alpha-D-galactose 1-phosphate | with UDP-glucose | Homo sapiens |  |
| 0.015 | - |
UDP-glucose | erythrocytes | Homo sapiens | |
| 0.021 | - |
alpha-D-galactose 1-phosphate | erythrocytes | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Homo sapiens |
| 67000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + alpha-D-galactose 1-phosphate | Homo sapiens | 2nd step of Leloir pathway | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
3 isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial from erythrocyte, several isozymes | Homo sapiens |
| placental enzyme to homogeneity, coelutes with a 67 kDa glycoprotein | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 44 | - |
erythrocytes | Homo sapiens |
| 92.2 | - |
purified enzyme from placenta | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + alpha-D-galactose 1-phosphate | - |
Homo sapiens | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | 2nd step of Leloir pathway | Homo sapiens | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Homo sapiens |
| More | a second band of MW 22 kDa is found in SDS-PAGE, which shows no enzyme activity | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| uridine diphosphoglucose:alpha-D-galactose-1-phosphate uridilyltransferase | - |
Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | 3 placental isozymes | 5.86 | 5.72 |
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Release 2023.1 (January 2023)
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