Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.102 extracted from

  • Yan, J.; Holzer, S.; Pellegrini, L.; Bell, S.D.
    An archaeal primase functions as a nanoscale caliper to define primer length (2018), Proc. Natl. Acad. Sci. USA, 115, 6697-6702 .
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97Z83 and Q9UWW1 Q97Z83 i.e. small subunit PriS, Q9UWW1 i.e large subunit PriL
-
Saccharolobus solfataricus DSM 1617 Q97Z83 and Q9UWW1 Q97Z83 i.e. small subunit PriS, Q9UWW1 i.e large subunit PriL
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3' + n dNTP
-
Saccharolobus solfataricus 5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3'/pppdN(pdN)n-1 + n-1 diphosphate
-
?
5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3' + n dNTP
-
Saccharolobus solfataricus DSM 1617 5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3'/pppdN(pdN)n-1 + n-1 diphosphate
-
?
5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3' + n NTP
-
Saccharolobus solfataricus 5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3'/pppN(dN)n-1 + n-1 diphosphate
-
?
5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3' + n NTP
-
Saccharolobus solfataricus DSM 1617 5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3'/pppN(dN)n-1 + n-1 diphosphate
-
?
additional information enzyme can synthesize DNA, RNA, and mixed DNA-RNA primers in vitro. At physiologically relevant ratios of substrate, the primase is almost exclusively a RNA synthetic enzyme. There is little discrimination in the initiation site for ATP over dATP, but the inclusion of dATP results in a general decrease of primer length Saccharolobus solfataricus ?
-
?
additional information enzyme can synthesize DNA, RNA, and mixed DNA-RNA primers in vitro. At physiologically relevant ratios of substrate, the primase is almost exclusively a RNA synthetic enzyme. There is little discrimination in the initiation site for ATP over dATP, but the inclusion of dATP results in a general decrease of primer length Saccharolobus solfataricus DSM 1617 ?
-
?