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Literature summary for 2.7.7.102 extracted from
- Characterization of a functional DnaG-type primase in archaea implications for a dual-primase system (2010), J. Mol. Biol., 397, 664-676 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E175G | complete loss of activity | Saccharolobus solfataricus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | or Mn2+, required. Optimum concentration 10 mM | Saccharolobus solfataricus |  |
| Mn2+ | or Mg2+, required. Optimum concentration 11 mM | Saccharolobus solfataricus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97Z83 and Q9UWW1 | Q97Z83 i. e. small subunit PriS, Q9UWW1 i.e. large subunit PriL | - |
| Saccharolobus solfataricus ATCC 35092 | Q97Z83 and Q9UWW1 | Q97Z83 i. e. small subunit PriS, Q9UWW1 i.e. large subunit PriL | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from insoluble recombinant aggregates, solubilization by addition of high concentrations of glycerol, polyethylene glycol, or Mn2+, and by incubation at 60°C | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bacteriophage M13 ssDNA + n NTP | - |
Saccharolobus solfataricus | bacteriophage M13 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate | - |
? | |
| bacteriophage M13 ssDNA + n NTP | - |
Saccharolobus solfataricus ATCC 35092 | bacteriophage M13 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Sso0079 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
poor activity below | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00013 | - |
NTP | pH 8.5, 70°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
50 mM Tris-HCl buffer | Saccharolobus solfataricus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00041 | - |
NTP | pH 8.5, 70°C | Saccharolobus solfataricus | |
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Release 2023.1 (January 2023)
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