Literature summary for 2.7.7.102 extracted from

Le Breton, M.; Henneke, G.; Norais, C.; Flament, D.; Myllykallio, H.; Querellou, J.; Raffin, J.
The heterodimeric primase from the euryarchaeon Pyrococcus abyssi a multifunctional enzyme for initiation and repair? (2007), J. Mol. Biol., 374, 1172-1185 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, independently and coexpression of subunits	Pyrococcus abyssi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5	-	dNTP	pH 8.0, 60°C, presence of 10 mM Mg2+	Pyrococcus abyssi
27.5	-	NTP	pH 8.0, 60°C, presence of 10 mM Mg2+	Pyrococcus abyssi
60.5	-	dNTP	pH 8.0, 60°C, presence of 5 mM Mg2+	Pyrococcus abyssi
198	-	dNTP	pH 8.0, 60°C, presence of 5 mM Mn2+, 10 mM Mg2+	Pyrococcus abyssi

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	or Mn2+, required. Optimum concentration 5 mM	Pyrococcus abyssi
Mn2+	or Mg2+, required. Optimum concentration 1 mM	Pyrococcus abyssi

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus abyssi	Q9V292 and Q9V291	Q9V292 i.e. small subunit PriS, Q9V291 i.e. large subunit PriL	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
M13 ssDNA + n dNTP	-	Pyrococcus abyssi	M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate	-	?
M13 ssDNA + n NTP	-	Pyrococcus abyssi	M13 ssDNA/pppN(pN)n-1 + (n-1) diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 41000, subunit PrimS, plus x * 46000, subunit PrimL, claculated from sequence	Pyrococcus abyssi

Synonyms

Synonyms	Comment	Organism
Pab2235	-	Pyrococcus abyssi
Pab2236	-	Pyrococcus abyssi

General Information

General Information	Comment	Organism
physiological function	the small subunit PrimS alone has no RNA synthesis activity but can synthesize up to 3 kb long DNA strands. Addition of the large subunit increases the rate of DNA synthesis but decreases the length of the DNA fragments synthesized and confers RNA synthesis capability. The primase has comparable affinities for ribonucleotides and deoxyribonucleotides. DNA primase also displays DNA polymerase, gapfilling, and strand-displacement activities	Pyrococcus abyssi

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Release 2023.1 (January 2023)