Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli BL21(DE3) | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
ATP | pH 7.0, 70°C | Pyrococcus horikoshii | |
0.061 | - |
nicotinamide ribonucleotide | pH 7.0, 70°C | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, the enzyme requires divalent cations for the activity. MgCl2 is the most effective additive. can replace Mg2+ with 51% of the activity compared to 10 mM Mg2+ | Pyrococcus horikoshii | |
Cu2+ | 10 mM, the enzyme requires divalent cations for the activity. MgCl2 is the most effective additive. can replace Mg2+ with 28% of the activity compared to 10 mM Mg2+ | Pyrococcus horikoshii | |
Mg2+ | 10 mM, the enzyme requires divalent cations for the activity. MgCl2 is the most effective additive. It can be replaced by CoCl2, ZnCl2, CuCl2, and MnCl2 to some extent | Pyrococcus horikoshii | |
Mn2+ | 10 mM, the enzyme requires divalent cations for the activity. MgCl2 is the most effective additive. can replace Mg2+ with 8% of the activity compared to 10 mM Mg2+ | Pyrococcus horikoshii | |
Zn2+ | 10 mM, the enzyme requires divalent cations for the activity. MgCl2 is the most effective additive. can replace Mg2+ with 30% of the activity compared to 10 mM Mg2+ | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
gel filtration | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Pyrococcus horikoshii OT-3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + nicotinamide ribonucleotide | at 70°C, ATP is a prominent donor. At 90°C the activity with ADP is 17.5% of the activity with ATP as substrate | Pyrococcus horikoshii | NAD+ + phosphate | - |
? | |
ADP + nicotinamide ribonucleotide | at 70°C, ATP is a prominent donor. At 90°C the activity with ADP is 17.5% of the activity with ATP as substrate | Pyrococcus horikoshii OT-3 | NAD+ + phosphate | - |
? | |
AMP + nicotinamide ribonucleotide | at 70°C, ATP is a prominent donor. At 90°C the activity with ADP is 50.6% of the activity with ATP as substrate | Pyrococcus horikoshii | NAD+ | - |
? | |
AMP + nicotinamide ribonucleotide | at 70°C, ATP is a prominent donor. At 90°C the activity with ADP is 50.6% of the activity with ATP as substrate | Pyrococcus horikoshii OT-3 | NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | at 70°C, ATP is a prominent donor. However, above 80°C, a relatively small, but significant, NMNAT activity is detected when ATP was replaced by ADP or AMP in the reaction mixture | Pyrococcus horikoshii | diphosphate + NAD+ | - |
? | |
ATP + nicotinamide ribonucleotide | at 70°C, ATP is a prominent donor. However, above 80°C, a relatively small, but significant, NMNAT activity is detected when ATP was replaced by ADP or AMP in the reaction mixture | Pyrococcus horikoshii OT-3 | diphosphate + NAD+ | - |
? | |
additional information | the following substrates are inert: GDP, CDP, UDP, IDP, GTP, CTP, ITP, IMP, CMP, TTP, and UTP | Pyrococcus horikoshii | ? | - |
? | |
additional information | the following substrates are inert: GDP, CDP, UDP, IDP, GTP, CTP, ITP, IMP, CMP, TTP, and UTP | Pyrococcus horikoshii OT-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 3 * or 4 * 21391, calculated from sequence | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
NMN adenylyltransferase | - |
Pyrococcus horikoshii |
NMNAT | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
30 min, stable | Pyrococcus horikoshii |
90 | - |
the enzyme loses 20% and 40% of its activity after 30 and 60 min incubation | Pyrococcus horikoshii |
100 | - |
100 min, complete inactivation | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pyrococcus horikoshii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | 70°C, 30 min, stable | Pyrococcus horikoshii |