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Literature summary for 2.7.7.1 extracted from

  • Sakuraba, H.; Kanai, K.; Goda, S.; Kawarabayasi, Y.; Ohshima, T.
    A nicotinamide mononucleotide adenylyltransferase with unique adenylyl group donor specificity from a hyperthermophilic archaeon, Pyrococcus horikoshii OT-3 (2003), J. Mol. Catal. B, 23, 273-279 .
No PubMed abstract available
Search for more literature for 2.7.7.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli BL21(DE3) Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 ATP pH 7.0, 70°C Pyrococcus horikoshii
0.061
-
 nicotinamide ribonucleotide pH 7.0, 70°C Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10 mM, the enzyme requires divalent cations for the activity. MgCl2 is the most effective additive. can replace Mg2+ with 51% of the activity compared to 10 mM Mg2+ Pyrococcus horikoshii
Cu2+ 10 mM, the enzyme requires divalent cations for the activity. MgCl2 is the most effective additive. can replace Mg2+ with 28% of the activity compared to 10 mM Mg2+ Pyrococcus horikoshii
Mg2+ 10 mM, the enzyme requires divalent cations for the activity. MgCl2 is the most effective additive. It can be replaced by CoCl2, ZnCl2, CuCl2, and MnCl2 to some extent Pyrococcus horikoshii
Mn2+ 10 mM, the enzyme requires divalent cations for the activity. MgCl2 is the most effective additive. can replace Mg2+ with 8% of the activity compared to 10 mM Mg2+ Pyrococcus horikoshii
Zn2+ 10 mM, the enzyme requires divalent cations for the activity. MgCl2 is the most effective additive. can replace Mg2+ with 30% of the activity compared to 10 mM Mg2+ Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
 gel filtration Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-
Pyrococcus horikoshii OT-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + nicotinamide ribonucleotide at 70°C, ATP is a prominent donor. At 90°C the activity with ADP is 17.5% of the activity with ATP as substrate Pyrococcus horikoshii NAD+ + phosphate
-
 ?
ADP + nicotinamide ribonucleotide at 70°C, ATP is a prominent donor. At 90°C the activity with ADP is 17.5% of the activity with ATP as substrate Pyrococcus horikoshii OT-3 NAD+ + phosphate
-
 ?
AMP + nicotinamide ribonucleotide at 70°C, ATP is a prominent donor. At 90°C the activity with ADP is 50.6% of the activity with ATP as substrate Pyrococcus horikoshii NAD+
-
 ?
AMP + nicotinamide ribonucleotide at 70°C, ATP is a prominent donor. At 90°C the activity with ADP is 50.6% of the activity with ATP as substrate Pyrococcus horikoshii OT-3 NAD+
-
 ?
ATP + nicotinamide ribonucleotide at 70°C, ATP is a prominent donor. However, above 80°C, a relatively small, but significant, NMNAT activity is detected when ATP was replaced by ADP or AMP in the reaction mixture Pyrococcus horikoshii diphosphate + NAD+
-
 ?
ATP + nicotinamide ribonucleotide at 70°C, ATP is a prominent donor. However, above 80°C, a relatively small, but significant, NMNAT activity is detected when ATP was replaced by ADP or AMP in the reaction mixture Pyrococcus horikoshii OT-3 diphosphate + NAD+
-
 ?
additional information the following substrates are inert: GDP, CDP, UDP, IDP, GTP, CTP, ITP, IMP, CMP, TTP, and UTP Pyrococcus horikoshii ?
-
 ?
additional information the following substrates are inert: GDP, CDP, UDP, IDP, GTP, CTP, ITP, IMP, CMP, TTP, and UTP Pyrococcus horikoshii OT-3 ?
-
 ?

Subunits

Subunits Comment Organism
? 3 * or 4 * 21391, calculated from sequence Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
NMN adenylyltransferase
-
 Pyrococcus horikoshii
NMNAT
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 30 min, stable Pyrococcus horikoshii
90
-
 the enzyme loses 20% and 40% of its activity after 30 and 60 min incubation Pyrococcus horikoshii
100
-
 100 min, complete inactivation Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pyrococcus horikoshii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 70°C, 30 min, stable Pyrococcus horikoshii