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Literature summary for 2.7.7.1 extracted from

  • Song, T.; Yang, L.; Kabra, N.; Chen, L.; Koomen, J.; Haura, E.B.; Chen, J.
    The NAD+ synthesis enzyme nicotinamide mononucleotide adenylyltransferase (NMNAT1) regulates ribosomal RNA transcription (2013), J. Biol. Chem., 288, 20908-20917.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene NMNAT1, recombinant expression in H-1299 and U2-O2 cells, recombinant co-expression of His6-tagged NMNAT1 and GST-tagged NML in Escherichia coli, co-expression of FLAG-tagged nucleomethylin and Myc-tagged NMNAT1 in H-1299 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information isozyme NMNAT1 knockdown by expression of NMNAT1 specific siRNA. NMNAT1 knockdown promotes glucose starvation-induced cell death Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens isozyme NMNAT1 directly interacts with nucleolar protein nucleomethylin, NML ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HAN9 gene Nmnat1
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Purification (Commentary)

Purification (Comment) Organism
co-purification of recombinant His6-tagged NMNAT1 and GST-tagged NML from Escherichia coli, His6-NMNAT1 is pulled down by beads loaded with GST-NML, but not by GST suggesting that the two proteins interact directly Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
A-427 cell low NMNAT1 expression level Homo sapiens
-
A-549 cell low NMNAT1 expression level Homo sapiens
-
H-1299 cell high NMNAT1 expression level Homo sapiens
-
H-1299 cell low NMNAT1 expression level Homo sapiens
-
H-1355 cell high NMNAT1 expression level Homo sapiens
-
H-1648 cell high NMNAT1 expression level Homo sapiens
-
H-1703 cell high NMNAT1 expression level Homo sapiens
-
H-1944 cell low NMNAT1 expression level Homo sapiens
-
H-2110 cell medium NMNAT1 expression level Homo sapiens
-
H-2172 cell high NMNAT1 expression level Homo sapiens
-
H-2228 cell high NMNAT1 expression level Homo sapiens
-
H-292 cell low NMNAT1 expression level Homo sapiens
-
H-358 cell medium NMNAT1 expression level Homo sapiens
-
H-441 cell high NMNAT1 expression level Homo sapiens
-
H-460 cell low NMNAT1 expression level Homo sapiens
-
H-820 cell high NMNAT1 expression level Homo sapiens
-
HCC-1171 cell medium NMNAT1 expression level Homo sapiens
-
HCC-2279 cell medium NMNAT1 expression level Homo sapiens
-
HCC-4006 cell low NMNAT1 expression level Homo sapiens
-
lung cancer cell NMNAT1 expression level is significantly reduced in a subset of lung tumor cell lines Homo sapiens
-
additional information isozyme NMNAT1 expression is down-regulated in a subset of human tumors, quantitative PCR expression analysis in 28 cell lines, overview Homo sapiens
-
PC-9 cell low NMNAT1 expression level Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information isozyme NMNAT1 directly interacts with nucleolar protein nucleomethylin, NML Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
nicotinamide mononucleotide adenylyltransferase
-
Homo sapiens
NMNAT1
-
Homo sapiens

Expression

Organism Comment Expression
Homo sapiens NMNAT1 expression is induced by DNA damage up

General Information

General Information Comment Organism
malfunction the chromosomal region encoding the nuclear NAD+ synthesis enzyme NMNAT1 is frequently deleted in human cancer, which may contribute to tumor development. Knockdown of NMNAT1 enhances rRNA transcription and promotes cell death after nutrient deprivation. Heterozygous deletion of NMNAT1 in lung tumor cell lines correlates with low expression level and increased sensitivity to DNA damage. NMNAT1 knockdown stimulates p53 acetylation level before and after DNA damage with doxorubicin Homo sapiens
metabolism NMNAT1 catalyzes NAD+ synthesis in the last step of a salvage synthesis pathway that recycles nicotinamide (NAM) back to NAD+. NMNAT1 deletion in tumors may contribute to transformation by increasing rRNA synthesis, but may also increase sensitivity to nutrient stress and DNA damage Homo sapiens
physiological function nicotinamide mononucleotide adenylyltransferase (NMNAT1) regulates ribosomal RNA transcription and plays a role in preventing cell death after damage. It interacts with the nucleolar repressor protein nucleomethylin. Isozyme NMNAT1 participates in the regulation of rRNA biosynthesis, possibly by producing a local supply of NAD+. And NMNAT1 may be regulated by recruitment into complexes that consume NAD+. The enzyme is recruited into a ternary complex containing the NAD+-dependent deacetylase SirT1. NMNAT1 expression stimulates the deacetylase function of SirT1 to deacetylate p53. Nucleolar protein nucleomethylin, NML, promotes interaction between SirT1 and NMNAT1, and NMNAT1 is recruited to the nucleolar transcriptional repressor complex eNoSC by NML Homo sapiens