Cloned (Comment) | Organism |
---|---|
gene NMNAT2, semi-quantitative and real-time PCR enzyme expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | H1299 human non-small lung cancer cell line, which lacks expression of endogenous p53 due to homozygous partial deletion of the p53 gene, is used to construct a stable cell line expressing wild-type p53 under the control of doxycycline. mRNA levels of NAD+ synthetic enzymes, including NAMPT, NMNAT1, NMNAT2, and NMNAT3, are compared in the presence or absence of p53 by semi-quantitative PCR: only the NMNAT2 mRNA level is significantly elevated by ectopic p53 expression. Although genes NMNAT1 and NMNAT3 are also induced, their expression levels are significantly lower than those of NMNAT?2 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Homo sapiens | 5794 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BZQ4 | gene NMNAT2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colon cancer cell | - |
Homo sapiens | - |
H-1299 cell | - |
Homo sapiens | - |
HCT-116 cell | - |
Homo sapiens | - |
non-small cell lung cancer cell | - |
Homo sapiens | - |
U2-OS cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
nicotinamide mononucleotide adenylyltransferase 2 | - |
Homo sapiens |
NMNAT-2 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | NMNAT-2 expression is induced upon DNA damage in a p53-dependent manner. Two putative p53 binding sites are identified within the human NMNAT-2 gene, and both are functional in a p53-dependent manner, overview. DNA damage agents, actinomycin D and doxorubicin, and Nutlin-3a, a p53 stabilizer that disrupts interaction of p53 with its negative regulator MDM2, in a time-dependent manner in control but not in p53-knockdown U2-OS cells | up |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of NMNAT-2 significantly reduces cellular NAD+ levels and protects cells from p53-dependent cell death upon DNA damage. p53 knockdown abolishes NMNAT-2 expression upon actinomycin D treatment. In NMNAT-2-knockdown cells, actinomycin D treatment does not result in enhanced immunoreactivity | Homo sapiens |
metabolism | gene expression of NAD+ synthesizing enzymes in the NAD+ salvage pathways can be modulated by p53 | Homo sapiens |
physiological function | the enzyme is a target for the tumor suppressor p53, a major player in cancer signaling pathways, that is an important regulator of cellular metabolism. Determination of an important functional role of NMNAT-2 in p53-mediated signaling. Enzyme NMNAT-2 plays an important role in p53-mediated cell death upon DNA damage | Homo sapiens |