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Literature summary for 2.7.7.1 extracted from

  • Magni, G.; Raffaelli, N.; Emanuelli, M.; Amici, A.; Natalini, P.; Ruggieri, S.
    Nicotinamide-mononucleotide adenylyltransferases from yeast and other microorganisms (1997), Methods Enzymol., 280, 248-255.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Saccharomyces cerevisiae
p-chloromercuribenzoate
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
ATP 70°C, pH 7.4 Saccharolobus solfataricus
0.0014
-
NMN 70°C, pH 7.4 Saccharolobus solfataricus
0.017
-
nicotinic acid mononucleotide 70°C, pH 7.4 Saccharolobus solfataricus
0.11
-
ATP
-
Saccharomyces cerevisiae
0.19
-
NMN
-
Saccharomyces cerevisiae
5
-
nicotinate ribonucleotide
-
Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus chromatin-associated Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Saccharomyces cerevisiae
Co2+
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol Saccharomyces cerevisiae
66000
-
gel filtration Saccharolobus solfataricus
195000
-
-
Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 2% carbohydrates Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.35
-
-
Saccharolobus solfataricus
2.8
-
purification step: Matrix Gel Green A Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, in 20 mM HEPES buffer, pH 7.5, about 20% loss of activity within 1 month Saccharomyces cerevisiae
-20°C, several weeks without loss in activity Saccharolobus solfataricus
4°C, in 20 mM HEPES buffer, pH 7.5, about 30% loss of activity within 2 weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Saccharomyces cerevisiae diphosphate + NAD+
-
r
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Saccharolobus solfataricus diphosphate + NAD+
-
r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Saccharomyces cerevisiae diphosphate + nicotinic acid-adenine dinucleotide
-
r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Saccharolobus solfataricus diphosphate + nicotinic acid-adenine dinucleotide
-
r

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.4
-
Saccharomyces cerevisiae

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus FPLC chromatofocusing on a Mono P HR 5/5 column
-
5.4
Saccharomyces cerevisiae in the presence of 4 M urea, multiple pIs in its absence at pH 4.2, 4.9 and 6.2
-
6.2