Literature summary for 2.7.7.1 extracted from

Emanuelli, M.; Carnevali, F.; Lorenzi, M.; Raffaelli, N.; Amici, A.; Ruggieri, S.; Magni, G.
Identification and characterization of YLR328W, the Saccharomyces cerevisiae structural gene encoding NMN adenylyltransferase. Expression and characterization of the recombinant enzyme (1999), FEBS Lett., 455, 13-17.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Hg2+	-	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
divalent cations	-	Saccharomyces cerevisiae
Ni2+	optimal activity at 5 mM	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	alpha4, 4 * 48000, SDS-PAGE, recombinant enzyme	Saccharomyces cerevisiae
195000	-	gel filtration	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
74.2	-	recombinant enzyme YLR328W, purification step: phenyl Superose	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + nicotinamide ribonucleotide	i.e. NMN or nicotinamide mononucleotide	Saccharomyces cerevisiae	diphosphate + NAD+	-	r

Subunits

Subunits	Comment	Organism
tetramer	alpha4, 4 * 48000, SDS-PAGE, recombinant enzyme	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)