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Literature summary for 2.7.6.5 extracted from

  • Kriel, A.; Brinsmade, S.R.; Tse, J.L.; Tehranchi, A.K.; Bittner, A.N.; Sonenshein, A.L.; Wang, J.D.
    GTP dysregulation in Bacillus subtilis cells lacking (p)ppGpp results in phenotypic amino acid auxotrophy and failure to adapt to nutrient downshift and regulate biosynthesis genes (2014), J. Bacteriol., 196, 189-201.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.6.5

Cloned(Commentary)

Cloned (Comment) Organism
gene relA, recombinant expression of wild-type and mutant enzymes in Escherichia coli Bacillus subtilis
gene yjbM, recombinant expression of wild-type and mutant enzymes in Escherichia coli Bacillus subtilis
gene ywaC, recombinant expression of wild-type and mutant enzymes in Escherichia coli Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D264G site-directed mutagenesis, the mutation specifically abolishes (p)ppGpp synthetase activity without affecting other potential functions of the protein. Loss of the (p)ppGpp synthetase activity results in failure to grow on minimal medium and requirement for valine, leucine, isoleucine, threonine, and methionine, and a weaker requirement for arginine, histidine, and tryptophan addition Bacillus subtilis
D72G site-directed mutagenesis, the mutation specifically abolishes (p)ppGpp synthetase activity without affecting other potential functions of the protein. Loss of the (p)ppGpp synthetase activity results in failure to grow on minimal medium and requirement for valine, leucine, isoleucine, threonine, and methionine, and a weaker requirement for arginine, histidine, and tryptophan addition Bacillus subtilis
D87G site-directed mutagenesis, the mutation specifically abolishes (p)ppGpp synthetase activity without affecting other potential functions of the protein. Loss of the (p)ppGpp synthetase activity results in failure to grow on minimal medium and requirement for valine, leucine, isoleucine, threonine, and methionine, and a weaker requirement for arginine, histidine, and tryptophan addition Bacillus subtilis
additional information generation of strain JDW1464 (relAD264G ywaCD87GyjbMD72G) by substituting a conserved aspartic acid residue in each of the three (p)ppGpp synthetases, corresponding to D264 in RelA, D87 in YwaC, and D72 in YjbM, with glycine Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + GTP Bacillus subtilis
-
 AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
ATP + GTP Bacillus subtilis 168
-
 AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O31611 gene yjbM
-
Bacillus subtilis O54408 gene relA
-
Bacillus subtilis P39583 gene ywaC
-
Bacillus subtilis 168 O31611 gene yjbM
-
Bacillus subtilis 168 O54408 gene relA
-
Bacillus subtilis 168 P39583 gene ywaC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GTP
-
 Bacillus subtilis AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
ATP + GTP
-
 Bacillus subtilis 168 AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?

Synonyms

Synonyms Comment Organism
(p)ppGpp synthase
-
 Bacillus subtilis
ATP:GTP 3'-pyrophosphotransferase
-
 Bacillus subtilis
ppGpp synthase I
-
 Bacillus subtilis
RelA
-
 Bacillus subtilis
SAS 1
-
 Bacillus subtilis
SAS 2
-
 Bacillus subtilis
small alarmone synthase 1
-
 Bacillus subtilis
small alarmone synthase 2
-
 Bacillus subtilis
YjbM
-
 Bacillus subtilis
YwaC
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bacillus subtilis

Expression

Organism Comment Expression
Bacillus subtilis the transcription factor CodY represses the ywaA operon, overview down
Bacillus subtilis CodY-independent activation of transcription of the ywaA operon, overview up

General Information

General Information Comment Organism
malfunction GTP dysregulation in Bacillus subtilis cells lacking (p)ppGpp results in phenotypic amino acid auxotrophy and failure to adapt to nutrient downshift and regulate biosynthesis genes. Loss of the (p)ppGpp synthetase activity results in a failure of Bacillus subtilis to grow on minimal medium and causes the requirement for valine, leucine, isoleucine, threonine, and methionine, and a weaker requirement for arginine, histidine, and tryptophan addition Bacillus subtilis
physiological function guanosine 3'-diphosphate 5'-triphosphate, (p)ppGpp, synthesis is required for amino acid prototrophy Bacillus subtilis