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Literature summary for 2.7.6.5 extracted from

  • Knutsson Jenvert, R.M.; Holmberg Schiavone, L.
    Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli (2005), FEBS J., 272, 685-695.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information addition of template, unacylated tRNA and ribosomes to the activity assay stimulates SF 30fold when using optimal conditions, activity of SF increases threefold in the presence of twice salt-washed tight-couple ribosomes and twofold in the presence of reassociated ribosomes compared to the endogenous activity of the enzyme Escherichia coli
unacylated tRNA in the ribosomal amino-acyl site (A-site) Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Escherichia coli

General Stability

General Stability Organism
the purified SF is stored in the freezer, at a concentration of 1.0 mg/ml in 20% (v/v) glycerol, without forming a precipitate or loss of activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
micrococcin ribosome-dependent activity Escherichia coli
tetracycline endogenous diphoyphoryl transferase activity; ribosome-dependent activity Escherichia coli
Thiostrepton ribosome-dependent activity Escherichia coli
viomycin ribosome-dependent activity Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the magnesium concentration also affects the binding states of the tRNAs on the ribosome Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + GTP Escherichia coli
-
AMP + guanosine 5'-triphosphate 3'-diphosphate
-
r
ATP + GTP Escherichia coli MRE 600
-
AMP + guanosine 5'-triphosphate 3'-diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
MRE 600
-
Escherichia coli MRE 600
-
MRE 600
-

Purification (Commentary)

Purification (Comment) Organism
affinity-chromatography and precipitation Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GTP
-
Escherichia coli AMP + guanosine 5'-triphosphate 3'-diphosphate
-
r
ATP + GTP
-
Escherichia coli MRE 600 AMP + guanosine 5'-triphosphate 3'-diphosphate
-
r

Synonyms

Synonyms Comment Organism
ATP:GTP pyrophosphoryl transferase
-
Escherichia coli
SF
-
Escherichia coli
stringent factor
-
Escherichia coli