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Literature summary for 2.7.6.4 extracted from

  • Oki, T.; Yoshimoto, A.; Sato, S.; Takamatsu, A.
    Purine nucleotide pyrophosphotransferase from Streptomyces morookaensis, capable of synthesizing pppApp and pppGpp (1975), Biochim. Biophys. Acta, 410, 262-272.
    View publication on PubMed
Search for more literature for 2.7.6.4

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 0.01 mg/ml, stimulates Streptomyces morookaense
Tween 40 0.01-0.001%, stimulate Streptomyces morookaense

General Stability

General Stability Organism
at low protein concentration, below 0.01 mg/ml, considerable loss in activity Streptomyces morookaense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 GDP pH 10.0, 37°C, reaction with pppApp Streptomyces morookaense
0.25
-
 GDP pH 10.0, 37°C, reaction with dATP Streptomyces morookaense
0.46
-
 GDP pH 10.0, 37°C, reaction with ATP Streptomyces morookaense
0.47
-
 GTP pH 10.0, 37°C, reaction with dATP Streptomyces morookaense
0.89
-
 GMP pH 10.0, 37°C, reaction with ATP Streptomyces morookaense
1.54
-
 GTP pH 10.0, 37°C, reaction with ATP Streptomyces morookaense
1.61
-
 ADP pH 10.0, 37°C, reaction with pppApp Streptomyces morookaense
1.67
-
 GTP pH 10.0, 37°C, reaction with pppApp Streptomyces morookaense
1.67
-
 ADP pH 10.0, 37°C, reaction with dATP Streptomyces morookaense
2.78
-
 AMP pH 10.0, 37°C, reaction with ATP Streptomyces morookaense
3.23
-
 ADP pH 10.0, 37°C, reaction with ATP Streptomyces morookaense

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ stimulates at an optimal concentration of 60 mM Streptomyces morookaense
Fe2+ 15% stimulation at the optimal concentration of 50-60 mM Streptomyces morookaense
Mg2+ most actively stimulates at 30-100 mM, half-maximal activation from 10 to 100 mM Streptomyces morookaense
Mn2+ half-maximal activation from 10 to 100 mM Streptomyces morookaense
Zn2+ 18% stimulation at the optimal concentration of 10-20 mM Streptomyces morookaense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 1 * 24000, SDS-PAGE Streptomyces morookaense
25000
-
 gel filtration Streptomyces morookaense

Organism

Organism UniProt Comment Textmining
Streptomyces morookaense
-
 ATCC19166
-
Streptomyces morookaense ATCC 19166
-
 ATCC19166
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces morookaense

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Streptomyces morookaense
-

Storage Stability

Storage Stability Organism
4°C, 0.05 M glycine/NaOH buffer, pH 9.0, 0.01 M MgCl2, in 60% saturated ammonium sulfate, 1 mg protein per mol, stable for at least several months Streptomyces morookaense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
 Streptomyces morookaense AMP + adenosine-3'-diphosphate-5'-triphosphate
-
 ?
ATP
-
 Streptomyces morookaense ATCC 19166 AMP + adenosine-3'-diphosphate-5'-triphosphate
-
 ?
ATP + ADP
-
 Streptomyces morookaense AMP + adenosine 3'-diphosphate 5'-diphosphate
-
 ?
ATP + ADP
-
 Streptomyces morookaense ATCC 19166 AMP + adenosine 3'-diphosphate 5'-diphosphate
-
 ?
ATP + AMP
-
 Streptomyces morookaense AMP + adenosine 3'-phosphate 5'-diphosphate
-
 ?
ATP + GDP
-
 Streptomyces morookaense AMP + guanosine 3'-diphosphate 5'-diphosphate
-
 ?
ATP + GDP
-
 Streptomyces morookaense ATCC 19166 AMP + guanosine 3'-diphosphate 5'-diphosphate
-
 ?
ATP + GMP
-
 Streptomyces morookaense AMP + guanosine 3'-phosphate 5'-diphosphate
-
 ?
ATP + GTP
-
 Streptomyces morookaense AMP + guanosine-3'-diphosphate-5'-triphosphate
-
 ?
ATP + GTP
-
 Streptomyces morookaense ATCC 19166 AMP + guanosine-3'-diphosphate-5'-triphosphate
-
 ?
dATP + ADP
-
 Streptomyces morookaense dAMP + adenosine 3'-diphosphate 5'-diphosphate
-
 ?
dATP + GDP
-
 Streptomyces morookaense dAMP + guanosine 3'-diphosphate 5'-diphosphate
-
 ?
dATP + GTP 40.2% of the activity with GTP as substrate Streptomyces morookaense dAMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
dATP + GTP 40.2% of the activity with GTP as substrate Streptomyces morookaense ATCC 19166 dAMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
pppApp + ADP
-
 Streptomyces morookaense ppApp + ATP
-
 ?
pppApp + GDP
-
 Streptomyces morookaense ppGpp + ATP
-
 ?
pppApp + GTP 32.2% of the activity with GTP as substrate Streptomyces morookaense pppGpp + ATP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 24000, SDS-PAGE Streptomyces morookaense

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 pH 7-11, 37°C, over 90% of the original activity is retained after 2 h Streptomyces morookaense
40
-
 10 min, 50% loss of original activity, inactivation is prevented by addition of substrate, non-ionic surface active agents or protein Streptomyces morookaense
100
-
 pH 9-11, in 0.05 M glycine/NaOH buffer containing 0.01 M MgCl2, no significant loss of activity after 15 min Streptomyces morookaense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 reaction activated by Fe2+ Streptomyces morookaense
9 9.5 reaction activated by Co2+ and Zn2+ Streptomyces morookaense
10
-
 above, reaction activated by Mg2+ or Mn2+ Streptomyces morookaense

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 37°C, 2 h, 90% of the original activity is retained Streptomyces morookaense

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces morookaense isoelectric focusing
-
 6.9