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Literature summary for 2.7.6.4 extracted from

  • Nishino, T.; Murao, S.
    Physicochemical and enzymatic properties of ATP:nucleotide pyrophosphotransferase (1975), Agric. Biol. Chem., 39, 1827-1833.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 98.5% inhibition Streptomyces adephospholyticus
KMnO4 1 mM, 76% inhibition Streptomyces adephospholyticus
Na-diphosphate 1 mM, 14% inhibition Streptomyces adephospholyticus
SDS 0.1%, 87% inhibition Streptomyces adephospholyticus
tetracyclin 0.5 mg/ml, 37% inhibition Streptomyces adephospholyticus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces adephospholyticus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ absolute requirement for a divalent metal ion, at pH 10.0 Mg2+ and Mn2+ are most effective, at pH 7.0 Mg2+, Co2+, Mn2+, Fe2+ and Zn2+ are effective Streptomyces adephospholyticus
Fe2+ absolute requirement for a divalent metal ion, at pH 10.0 Mg2+ and Mn2+ are most effective, at pH 7.0 Mg2+, Co2+, Mn2+, Fe2+ and Zn2+ are effective Streptomyces adephospholyticus
Mg2+ absolute requirement for a divalent metal ion, at pH 10.0, Mg2+ and Mn2+ are most effective. At pH 7.0, Mg2+, Co2+, Mn2+, Fe2+ and Zn2+ are effective Streptomyces adephospholyticus
Mn2+ absolute requirement for a divalent metal ion, at pH 10.0 Mg2+ and Mn2+ are most effective, at pH 7.0 Mg2+, Co2+, Mn2+, Fe2+ and Zn2+ are effective Streptomyces adephospholyticus
Zn2+ absolute requirement for a divalent metal ion, at pH 10.0 Mg2+ and Mn2+ are most effective, at pH 7.0 Mg2+, Co2+, Mn2+, Fe2+ and Zn2+ are effective Streptomyces adephospholyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
1 * 28000, SDS-PAGE Streptomyces adephospholyticus
29000
-
equilibrium sedimentation Streptomyces adephospholyticus

Organism

Organism UniProt Comment Textmining
Streptomyces adephospholyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
Streptomyces adephospholyticus AMP + adenosine-3'-diphosphate-5'-triphosphate
-
?
ATP + ADP
-
Streptomyces adephospholyticus AMP + adenosine 3'-diphosphate 5'-diphosphate
-
?
ATP + GDP
-
Streptomyces adephospholyticus AMP + guanosine 3'-diphosphate 5'-diphosphate
-
?
ATP + GMP
-
Streptomyces adephospholyticus AMP + guanosine 3'-phosphate 5'-diphosphate
-
?
ATP + GTP
-
Streptomyces adephospholyticus AMP + guanosine-3'-diphosphate-5'-triphosphate
-
?
ATP + IDP
-
Streptomyces adephospholyticus AMP + inosine 3'-diphosphate 5'-diphosphate
-
?
ATP + IMP
-
Streptomyces adephospholyticus AMP + inosine 3'-diphosphate 5'-phosphate
-
?
ATP + ITP
-
Streptomyces adephospholyticus AMP + inosine 3'-diphosphate 5'-triphosphate
-
?
dATP + ADP
-
Streptomyces adephospholyticus dAMP + adenosine 3'-diphosphate 5'-diphosphate
-
?
dATP + ATP
-
Streptomyces adephospholyticus dAMP + adenosine 3'-diphosphate 5'-triphosphate
-
?
dATP + GDP
-
Streptomyces adephospholyticus dAMP + guanosine 3'-diphosphate 5'-diphosphate
-
?
dATP + GMP
-
Streptomyces adephospholyticus dAMP + guanosine 3'-diphosphate 5'-phosphate
-
?
dATP + GTP
-
Streptomyces adephospholyticus dAMP + guanosine 3'-diphosphate 5'-triphosphate
-
?
dATP + IDP
-
Streptomyces adephospholyticus dAMP + inosine 3'-diphosphate 5'-triphosphate
-
?
dATP + IMP
-
Streptomyces adephospholyticus dAMP + inosine 3'-diphosphate 5'-phosphate
-
?
dATP + ITP
-
Streptomyces adephospholyticus dAMP + inosine 3'-diphosphate 5'-triphosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 28000, SDS-PAGE Streptomyces adephospholyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8 optimal pH with Co2+ as stimulating cation Streptomyces adephospholyticus
10 10.5 optimal pH with Mg2+ or Mn2+ as stimulating cation Streptomyces adephospholyticus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces adephospholyticus isoelectric focusing
-
7.7