Crystallization (Comment) | Organism |
---|---|
X-ray diffraction crystal structure analysis of both the SaHPPK/inhibitor/cofactor analogue ternary and the SaHPPK/cofactor analogue binary complexes | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-mercaptoguanine | - |
Staphylococcus aureus | |
additional information | structure-based design and development of functionalized mercaptoguanine derivatives as inhibitors of the folate biosynthesis pathway enzyme 6-hydroxymethyl-7,8-dihydropterin diphosphokinase from Staphylococcus aureus, binding of an S8-functionalized derivative of 8-mercaptoguanine, overview. Synthesis and binding analysis of a set of analogues including a compound displaying over 20fold higher affinity for SaHPPK than 8-mercaptoguanine. A number of the compounds exhibit low micromolar affinity for dihydropteroate synthase (DHPS, EC 2.5.1.15), the adjacent, downstream enzyme to HPPK, and may thus represent bienzyme inhibitors | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine | Staphylococcus aureus | - |
AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine | - |
Staphylococcus aureus | AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase | - |
Staphylococcus aureus |
HPPK | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the folate biosynthesis pathway catalyzing the diphosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin | Staphylococcus aureus |