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Literature summary for 2.7.6.1 extracted from

  • Kadziola, A.; Jepsen, C.H.; Johansson, E.; McGuire, J.; Larsen, S.; Hove-Jensen, B.
    Novel class III phosphoribosyl diphosphate synthase: structure and properties of the tetrameric, phosphate-activated, non-allosterically inhibited enzyme from Methanocaldococcus jannaschii (2005), J. Mol. Biol., 354, 815-828.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate activates, maximal activity at 190 mM phosphate Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion technique Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
ADP a single ADP binding site, the active site, is present per subunit Methanocaldococcus jannaschii
alpha,beta-methylene ATP
-
Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
ATP pH and temperature not specified in the publication Methanocaldococcus jannaschii
2.8
-
D-ribose 5-phosphate pH and temperature not specified in the publication Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the activity of the enzyme depends on the presence of divalent cations, of which Mg2+ is the most effective. Mn2+ is also accepted, but the activity is less than 15% of that obtained with Mg2+, when assayed at pH 9.5 Methanocaldococcus jannaschii
Mn2+ the activity of the enzyme depends on the presence of divalent cations, of which Mg2+ is the most effective. Mn2+ is also accepted, but the activity is less than 15% of that obtained with Mg2+, when assayed at pH 9.5 Methanocaldococcus jannaschii
additional information the enzyme is essentially inactive in the presence of Cu2+, Ca2+, Co2+, Ni2+ or Fe2+ Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32229
-
4 * 32229, calculated from sequence Methanocaldococcus jannaschii
147000
-
gel filtration Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58761
-
-
Methanocaldococcus jannaschii DSM 2661 Q58761
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Storage Stability

Storage Stability Organism
4°C or -20°C in 25 mM Tris–HCl (pH 7.6), 50% (v/v) glycerol, purified enzyme is stable for at least 90 days when stored at Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate the enzyme accepts ATP and dATP equally well as diphosphoryl donor Methanocaldococcus jannaschii AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
ATP + D-ribose 5-phosphate the enzyme accepts ATP and dATP equally well as diphosphoryl donor Methanocaldococcus jannaschii DSM 2661 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
dATP + D-ribose 5-phosphate the enzyme accepts ATP and dATP equally well as diphosphoryl donor Methanocaldococcus jannaschii dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
dATP + D-ribose 5-phosphate the enzyme accepts ATP and dATP equally well as diphosphoryl donor Methanocaldococcus jannaschii DSM 2661 dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
additional information the enzyme is essentially unable to use GTP (0.4% of the activity obtained with ATP), UTP (0.5%) or CTP (3%) as phosphoryl donor Methanocaldococcus jannaschii ?
-
?
additional information the enzyme is essentially unable to use GTP (0.4% of the activity obtained with ATP), UTP (0.5%) or CTP (3%) as phosphoryl donor Methanocaldococcus jannaschii DSM 2661 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 32229, calculated from sequence Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
phosphoribosyl diphosphate synthase
-
Methanocaldococcus jannaschii
PRPP synthase
-
Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Methanocaldococcus jannaschii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
72 95 70% of maximal activity at 72°C and 95°C Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
at pH 9.0, half-life: 8 min Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Methanocaldococcus jannaschii

pH Range

pH Minimum pH Maximum Comment Organism
10
-
the activity profile declines steeply above pH 10. At least in part, this reduction in activity at high pH values may be caused by the formation of a magnesium phosphate precipitate, and consequently cause Mg2+ depletion. At lower pH the activity increases more or less linearly from pH 6.0 to pH 9.5 Methanocaldococcus jannaschii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.057
-
ADP pH and temperature not specified in the publication Methanocaldococcus jannaschii
0.85
-
alpha,beta-methylene ATP pH and temperature not specified in the publication Methanocaldococcus jannaschii