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Literature summary for 2.7.6.1 extracted from

  • Zakataeva, N.P.; Romanenkov, D.V.; Skripnikova, V.S.; Vitushkina, M.V.; Livshits, V.A.; Kivero, A.D.; Novikova, A.E.
    Wild-type and feedback-resistant phosphoribosyl pyrophosphate synthetases from Bacillus amyloliquefaciens: purification, characterization, and application to increase purine nucleoside production (2012), Appl. Microbiol. Biotechnol., 93, 2023-2033.
    View publication on PubMed
Search for more literature for 2.7.6.1

Activating Compound

Activating Compound Comment Organism Structure
phosphate
-
 Bacillus amyloliquefaciens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus amyloliquefaciens

Protein Variants

Protein Variants Comment Organism
D58H the mutant exhibits nearly identical sensitivity to ADP and GDP as the wild-type protein and has a notably greater phosphate requirement for activation Bacillus amyloliquefaciens
L135I the mutation releases the enzyme from ADP and GDP inhibition and significantly increases its sensitivity to inorganic phosphate activation Bacillus amyloliquefaciens
N120S the mutation releases the enzyme from ADP and GDP inhibition and significantly increases its sensitivity to inorganic phosphate activation Bacillus amyloliquefaciens

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Bacillus amyloliquefaciens
GDP
-
 Bacillus amyloliquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 ATP wild type enzyme, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens
0.06
-
 ATP mutant enzyme N120S, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens
0.083
-
 ATP mutant enzyme D58H, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens
0.094
-
 ATP mutant enzyme L135I, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens
0.105
-
 D-ribose 5-phosphate wild type enzyme, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens
0.157
-
 D-ribose 5-phosphate mutant enzyme D58H, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens
0.18
-
 D-ribose 5-phosphate mutant enzyme N120S, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens
0.189
-
 D-ribose 5-phosphate mutant enzyme L135I, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
186000
-
 gel filtration Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens E7EAU9
-
-
Bacillus amyloliquefaciens IAM 1523 E7EAU9
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Sephadex G-25 gel filtration Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
 Bacillus amyloliquefaciens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
ATP + D-ribose 5-phosphate
-
 Bacillus amyloliquefaciens IAM 1523 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
phosphoribosyl pyrophosphate synthetase
-
 Bacillus amyloliquefaciens
PRS
-
 Bacillus amyloliquefaciens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.07
-
 mutant enzyme D58H, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens ADP
0.08
-
 wild type enzyme, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens ADP
0.44
-
 mutant enzyme N120S, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens ADP
0.45
-
 wild type enzyme, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens GDP
0.51
-
 mutant enzyme D58H, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens GDP
0.84
-
 mutant enzyme L135I, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens ADP
1
-
 IC50 above 1 mM, mutant enzyme L135I, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens GDP
1
-
 IC50 above 1 mM, mutant enzyme N120S, in 50 mM potassium phosphate at pH 7.5, 5 mM MgCl2, at 37°C Bacillus amyloliquefaciens GDP