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Literature summary for 2.7.6.1 extracted from

  • Hove-Jensen, B.; McGuire, J.N.
    Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase (2004), Eur. J. Biochem., 271, 4526-4533.
    View publication on PubMed
Search for more literature for 2.7.6.1

Activating Compound

Activating Compound Comment Organism Structure
phosphate required, maximal activity at 50 mM, slowly reduced to 58% at 120 mM and 17% at 200 mM [Bacillus] caldolyticus
SO42- can replace phosphate at about 30% of the maximal activity at a concentration of 0.5 M [Bacillus] caldolyticus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli [Bacillus] caldolyticus

Inhibitors

Inhibitors Comment Organism Structure
ADP at low concentrations ADP inhibition is linearly competitive with respect to ATP. Less than 1% residual activity in presence of 5 mM ADP [Bacillus] caldolyticus
GDP 5 mM, less than 1% residual activity [Bacillus] caldolyticus
SO42- 1 M, strong inhibition [Bacillus] caldolyticus
UTP 5.0 mM, significant inhibition [Bacillus] caldolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 ATP
-
 [Bacillus] caldolyticus
0.53
-
 D-ribose 5-phosphate
-
 [Bacillus] caldolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activity is 5-10% of the activity with Mg2+ [Bacillus] caldolyticus
Cu2+ activity is 5-10% of the activity with Mg2+ [Bacillus] caldolyticus
Mg2+ free Mg2+ required for maximal activity [Bacillus] caldolyticus
Mn2+ substitutes for Mg2+ at 30% of the activity obtained with Mg2+ [Bacillus] caldolyticus
Zn2+ activity is 5-10% of the activity with Mg2+ [Bacillus] caldolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34497
-
 6 * 34497, MALDI-TOF [Bacillus] caldolyticus

Organism

Organism UniProt Comment Textmining
[Bacillus] caldolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Bacillus] caldolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
440
-
-
 [Bacillus] caldolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
 [Bacillus] caldolyticus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 34497, MALDI-TOF [Bacillus] caldolyticus

Synonyms

Synonyms Comment Organism
5-phospho-alpha D-ribosyl 1-diphosphate synthase
-
 [Bacillus] caldolyticus
PRibPP synthase
-
 [Bacillus] caldolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65
-
 [Bacillus] caldolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 half-life: 26 min [Bacillus] caldolyticus
65
-
 half-life: 60 s [Bacillus] caldolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3 8.8
-
 [Bacillus] caldolyticus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH 6.5: about 25% of maximal activity, pH 9.5: about 80% of maximal activity [Bacillus] caldolyticus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.113
-
 ADP
-
 [Bacillus] caldolyticus
0.49
-
 GDP
-
 [Bacillus] caldolyticus