Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | isoenzyme 3 is independent of phosphate | Spinacia oleracea |
Cloned (Comment) | Organism |
---|---|
expression of isoenzyme 3 in Escherichia coli | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate | competitive with respect to ATP and noncompetitive with respect to D-ribose 5-phosphate | Spinacia oleracea | |
ADP | - |
Spinacia oleracea | |
AMP | noncompetitive to both substrates | Spinacia oleracea | |
GDP | - |
Spinacia oleracea | |
GTP | substrate inhibition | Spinacia oleracea | |
L-histidine | no inhibition | Spinacia oleracea | |
L-tryptophan | no inhibition | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
D-ribose 5-phosphate | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
0.116 | - |
CTP | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
0.137 | - |
UTP | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
0.17 | - |
ATP | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
0.233 | - |
dATP | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea | |
0.65 | - |
GTP | pH 7.6, 37ºC, isoenzyme 3 | Spinacia oleracea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | no activity observed | Spinacia oleracea | |
Co2+ | no activity observed | Spinacia oleracea | |
Mg2+ | requirement | Spinacia oleracea | |
Mg2+ | required to form a complex with ATP and as a free cation | Spinacia oleracea | |
Mn2+ | can partially replace Mg2+ | Spinacia oleracea | |
additional information | no activity observed with Ca2+ | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Spinacia oleracea | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
spinach | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate and polyethylene glycol precipitation, affinity chromatography and anion exchange chromatography, purification of isoenzyme 3 | Spinacia oleracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi-bi reaction | Spinacia oleracea | |
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | sequential kinetic mechanism | Spinacia oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.1 | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Spinacia oleracea | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
CTP + D-ribose 5-phosphate | less effective than ATP | Spinacia oleracea | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
dATP + D-ribose 5-phosphate | equally effective as ATP | Spinacia oleracea | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
GTP + D-ribose 5-phosphate | less effective than ATP | Spinacia oleracea | GMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
additional information | 2-deoxyribose-5-phosphate is not substrate | Spinacia oleracea | ? | - |
? | |
UTP + D-ribose 5-phosphate | less effective than ATP | Spinacia oleracea | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Spinacia oleracea |
40 | - |
- |
Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
activity increases up to pH 7.2 and decreases above pH 7.6 | Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
AMP | pH 7.6, 37ºC, 5-phospho-alpha-D-ribose 1-diphosphate as substrate, isoenzyme 3 | Spinacia oleracea | |
0.52 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.6, 37ºC, ATP as substrate, isoenzyme 3 | Spinacia oleracea | |
0.68 | - |
ADP | pH 7.6, 37ºC, ATP as substrate, isoenzyme 3 | Spinacia oleracea | |
0.82 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.6, 37ºC, 5-phospho-alpha-D-ribose 1-diphosphate as substrate, isoenzyme 3 | Spinacia oleracea | |
1.22 | - |
ADP | pH 7.6, 37ºC, 5-phospho-alpha-D-ribose 1-diphosphate as substrate, isoenzyme 3 | Spinacia oleracea | |
1.62 | - |
AMP | pH 7.6, 37ºC, ATP as substrate, isoenzyme 3 | Spinacia oleracea |