Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | specific and absolute requirement | Homo sapiens | |
phosphate | specific and absolute requirement | Rattus norvegicus | |
sulfate | can partially replace phosphate in activation | Homo sapiens | |
sulfate | can partially replace phosphate in activation | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of isoenzymes PRSI and PRSII in Escherichia coli | Homo sapiens |
expression of isoenzymes PRSI and PRSII in Escherichia coli | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Homo sapiens | |
ADP | one of the most effective | Rattus norvegicus | |
AMP | - |
Homo sapiens | |
AMP | one of the most effective | Rattus norvegicus | |
CTP | - |
Homo sapiens | |
CTP | weak inhibition at 1 mM | Rattus norvegicus | |
GDP | - |
Homo sapiens | |
GDP | one of the most effective | Rattus norvegicus | |
GTP | - |
Homo sapiens | |
GTP | weak inhibition at 1 mM | Rattus norvegicus | |
PRPP synthetase-associated proteins | inhibit catalytic and perhaps regulatory functions of the enzyme | Homo sapiens | |
PRPP synthetase-associated proteins | inhibit catalytic and perhaps regulatory functions of the enzyme | Rattus norvegicus | |
UDP | 10%-16% inhibition at 1 mM | Homo sapiens | |
UDP | 10%-16% inhibition at 1 mM | Rattus norvegicus | |
UMP | 10%-18% inhibition at 1 mM | Homo sapiens | |
UMP | 10%-18% inhibition at 1 mM | Rattus norvegicus | |
UTP | weak inhibition at 1 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
D-ribose 5-phosphate | pH 7.4, 37ºC, isoenzyme rPRSI | Rattus norvegicus | |
0.044 | - |
ATP | pH 7.4, 37ºC, isoenzyme rPRSI | Rattus norvegicus | |
0.06 | - |
ATP | pH 7.4, 37ºC, isoenzyme rPRSII | Rattus norvegicus | |
0.073 | - |
D-ribose 5-phosphate | pH 7.4, 37ºC, isoenzyme rPRSII | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
brush border | intestinal mucosa | Rattus norvegicus | 5903 | - |
cytosol | 60% of the total activity, fractionation by discontinuous sucrose density gradient centrifugation | Rattus norvegicus | 5829 | - |
microsome | 30% of the total activity, fractionation by discontinuous sucrose density gradient centrifugation | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
additional information | - |
the catalytic subunit complexes with the PRPP synthetase-associated protein of 39000 Da, PAP39, to form highly aggregated forms | Rattus norvegicus |
34000 | - |
x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII | Rattus norvegicus |
39000 | - |
x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII | Rattus norvegicus |
41000 | - |
x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII | Rattus norvegicus |
68000 | - |
x * 68000, gel filtration in the presence of MgCl2 1 M | Rattus norvegicus |
1000000 | - |
- |
Homo sapiens |
1000000 | - |
chromatography on DEAE-Toyopearl | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Homo sapiens | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + D-ribose 5-phosphate | Rattus norvegicus | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
rat | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | isoenzymes PRSI and PRSII | Rattus norvegicus | - |
adrenal gland | isoenzyme PRSI | Rattus norvegicus | - |
brain | isoenzyme PRSI | Rattus norvegicus | - |
erythrocyte | - |
Homo sapiens | - |
glioblastoma cell | cell line | Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
lung | isoenzyme PRSII | Rattus norvegicus | - |
spleen | isoenzyme PRSII | Rattus norvegicus | - |
testis | isoenzymes PRSI and PRSII | Rattus norvegicus | - |
thymus | isoenzymes PRSI and PRSII | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
additional information | - |
33400 U/mg and 46200 U/mg for PRSI and PRSII purified isoenzymes, respectively | Rattus norvegicus |
additional information | - |
purified enzyme has an activity of 7280 U/mg | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + D-ribose 5-phosphate | - |
Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | - |
Homo sapiens |
? | x * 34000 + x * 39000 + x * 41000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit which appears as PRSI or PRSII | Rattus norvegicus |
? | x * 68000, gel filtration in the presence of MgCl2 1 M | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49 | - |
- |
Homo sapiens |
49 | - |
half-life of 250 min, 90 min and 0.5 min for native enzyme, isoenzymes rPRSI and rPRSII, respectively | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | isoenzyme rPRSII | 4.4 | 4.3 |
Rattus norvegicus | isoenzyme rPRSI | - |
4.4 |