Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | requirement | Rattus norvegicus | |
phosphate | optimal activity at 100 mM, slight stabilizing effect | Rattus norvegicus |
General Stability | Organism |
---|---|
addition of albumin, EDTA or sulfhydryl compounds to the assay prevents loss of activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-ribose 5-phosphate | substrate inhibition above 1.5 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
ATP | pH 7.6, 37ºC, 100 mM phosphate | Rattus norvegicus | |
0.078 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
0.22 | - |
ATP | pH 7.6, 37ºC, excess of Mg2+ | Rattus norvegicus | |
0.22 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus | |
0.29 | - |
5-phospho-D-ribose diphosphate | pH 7.6, 37ºC | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
Mg2+ | requirement | Rattus norvegicus | |
Mg2+ | required to form a complex with ATP and as a free cation | Rattus norvegicus | |
Mg2+ | the most effective | Rattus norvegicus | |
Mn2+ | can partially replace Mg2+ | Rattus norvegicus | |
additional information | absolute requirement for a divalent cation for activity | Rattus norvegicus | |
additional information | no activity observed with Ca2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
40500 | - |
x * 40500, disc gel electrophoresis in SDS | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Rattus norvegicus | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
acetone powder, acid and streptomycin precipitation, ammonium sulfate fractionation, heat treatment and agarose gel filtration, 3400fold purification, presence of substrates enhances stability during purification | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
4ºC, 50% loss of activity after 2 months. -20ºC, more rapid loss of activity. Presence of substrates enhances stability during storage | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
CTP + D-ribose 5-phosphate | - |
Rattus norvegicus | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
dATP + D-ribose 5-phosphate | slightly more effective than ATP | Rattus norvegicus | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
additional information | ribose, ribose-1-phosphate, deoxyribose-5-phosphate, glucose-6-phosphate are not effective as substrates | Rattus norvegicus | ? | - |
? | |
UTP + D-ribose 5-phosphate | - |
Rattus norvegicus | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 40500, disc gel electrophoresis in SDS | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.8 | - |
Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
the enzyme retains over 70% of the activity | Rattus norvegicus |