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Literature summary for 2.7.6.1 extracted from

  • Roth, D.G.; Shelton, E.; Deuel, T.F.
    Purification and properties of phosphoribosyl pyrophosphate synthetase from rat liver (1974), J. Biol. Chem., 249, 291-296.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate requirement Rattus norvegicus
phosphate optimal activity at 100 mM, slight stabilizing effect Rattus norvegicus

General Stability

General Stability Organism
addition of albumin, EDTA or sulfhydryl compounds to the assay prevents loss of activity Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
D-ribose 5-phosphate substrate inhibition above 1.5 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
ATP pH 7.6, 37ºC, 100 mM phosphate Rattus norvegicus
0.078
-
ATP ATP in form of MgATP2- Rattus norvegicus
0.22
-
ATP pH 7.6, 37ºC, excess of Mg2+ Rattus norvegicus
0.22
-
ATP ATP in form of MgATP2- Rattus norvegicus
0.29
-
5-phospho-D-ribose diphosphate pH 7.6, 37ºC Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ in activation Rattus norvegicus
Mg2+ requirement Rattus norvegicus
Mg2+ required to form a complex with ATP and as a free cation Rattus norvegicus
Mg2+ the most effective Rattus norvegicus
Mn2+ can partially replace Mg2+ Rattus norvegicus
additional information absolute requirement for a divalent cation for activity Rattus norvegicus
additional information no activity observed with Ca2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
Rattus norvegicus
40500
-
x * 40500, disc gel electrophoresis in SDS Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-ribose 5-phosphate Rattus norvegicus the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
rat
-

Purification (Commentary)

Purification (Comment) Organism
acetone powder, acid and streptomycin precipitation, ammonium sulfate fractionation, heat treatment and agarose gel filtration, 3400fold purification, presence of substrates enhances stability during purification Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
4ºC, 50% loss of activity after 2 months. -20ºC, more rapid loss of activity. Presence of substrates enhances stability during storage Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
CTP + D-ribose 5-phosphate
-
Rattus norvegicus CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
dATP + D-ribose 5-phosphate slightly more effective than ATP Rattus norvegicus dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
additional information ribose, ribose-1-phosphate, deoxyribose-5-phosphate, glucose-6-phosphate are not effective as substrates Rattus norvegicus ?
-
?
UTP + D-ribose 5-phosphate
-
Rattus norvegicus UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Subunits

Subunits Comment Organism
? x * 40500, disc gel electrophoresis in SDS Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.8
-
Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
the enzyme retains over 70% of the activity Rattus norvegicus