Any feedback?
Literature summary for 2.7.6.1 extracted from
- Phosphoribosylpyrophosphate synthetase and related pyrophosphokinases (1974), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 10, 607-629.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | prevents inhibition by traces of cations | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| phosphate | activates at low concentrations | Salmonella enterica subsp. enterica serovar Typhimurium | |
| phosphate | specific and absolute requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
| phosphate | specific and absolute requirement | Mus musculus | |
| phosphate | specific and absolute requirement | Homo sapiens | |
| phosphate | specific and absolute requirement | Rattus norvegicus | |
| phosphate | appears to have an effect on the stability of the enzyme as well as a specific catalytic effect | Salmonella enterica subsp. enterica serovar Typhimurium | |
| phosphate | appears to have an effect on the stability of the enzyme as well as a specific catalytic effect | Homo sapiens | |
General Stability
| General Stability | Organism |
|---|---|
| purified enzyme, quite stable | Salmonella enterica subsp. enterica serovar Typhimurium |
| sulfhydryl compounds inactivate highly purified PRPP synthetase from erythrocytes, essential for stability and assay of partially purified enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-diphosphoglycerate | competitive to D-ribose 5-phosphate; inhibition of the erythrocyte enzyme | Homo sapiens | |
| ADP | - |
Columba sp. | |
| ADP | - |
Escherichia coli | |
| ADP | - |
Gallus gallus | |
| ADP | competitive inhibition to ATP | Homo sapiens | |
| ADP | - |
Mus musculus | |
| ADP | - |
Ophiodon elongatus | |
| ADP | competitive inhibition to ATP | Rattus norvegicus | |
| ADP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| alpha,beta-methyleneATP | competitive inhibition to ATP | Homo sapiens | |
| alpha,beta-methyleneATP | competitive inhibition to ATP | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Ca2+ | inhibition even in presence of excess Mg2+ | Escherichia coli | |
| Ca2+ | - |
Homo sapiens | |
| Ca2+ | inhibition even in presence of excess Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| D-ribose 5-phosphate | - |
Escherichia coli | |
| D-ribose 5-phosphate | - |
Rattus norvegicus | |
| D-ribose 5-phosphate | inhibition in presence of Ca2+, without ADP; substrate inhibition in presence of ADP, not in its absence | Salmonella enterica subsp. enterica serovar Typhimurium | |
| L-histidine | weak inhibition or no inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
| L-tryptophan | weak inhibition or no inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
| additional information | - |
Homo sapiens | |
| additional information | - |
Rattus norvegicus | |
| additional information | purines are more effective than pyrimidines, nucleoside tri and diphosphates are more effective than monophosphates, ribonucleotides are more effective than deoxynucleotides | Salmonella enterica subsp. enterica serovar Typhimurium | |
| nucleotides | - |
Rattus norvegicus | |
| nucleotides | nucleoside tri- and diphosphates are more effective than nucleoside monophosphates, ribonucleotides more effective than deoxynucleotides; purines more effective than pyrimidines | Salmonella enterica subsp. enterica serovar Typhimurium | |
| phosphate | inhibition at high concentrations, more pronounced in the reverse reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | increasing phosphate concentrations, increases Km values for ATP and D-ribose-5-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.014 | - |
ATP | pH and temperature conditions not mentioned | Homo sapiens | |
| 0.014 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
| 0.033 | - |
ribose 5-phosphate | pH and temperature conditions not mentioned | Homo sapiens | |
| 0.046 | - |
ATP | pH and temperature conditions not mentioned | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.046 | - |
ATP | ATP in form of MgATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.16 | - |
D-ribose 5-phosphate | pH and temperature conditions not mentioned | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.2 | - |
Mg2+ | pH and temperature conditions not mentioned | Homo sapiens | |
| 0.6 | - |
Mg2+ | pH and temperature conditions not mentioned | Salmonella enterica subsp. enterica serovar Typhimurium | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | 80% of the liver enzyme is found in the mitochondria | Columba sp. | 5739 | - |
| nucleus | 15% of the liver enzyme is found in the nucleus | Columba sp. | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Gallus gallus | |
| Mg2+ | - |
Mus musculus | |
| Mg2+ | - |
Escherichia coli | |
| Mg2+ | - |
Ophiodon elongatus | |
| Mg2+ | - |
Columba sp. | |
| Mg2+ | required to form a complex with ATP and as a free cation | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mg2+ | required to form a complex with ATP and as a free cation | Homo sapiens | |
| Mg2+ | required to form a complex with ATP and as a free cation | Rattus norvegicus | |
| Mg2+ | the most effective | Homo sapiens | |
| Mg2+ | the most effective | Rattus norvegicus | |
| Mn2+ | equally effective as Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
| additional information | absolute requirement for a divalent cation for activity | Salmonella enterica subsp. enterica serovar Typhimurium | |
| additional information | absolute requirement for a divalent cation for activity | Homo sapiens | |
| additional information | absolute requirement for a divalent cation for activity | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| additional information | - |
enzyme occurs in multiple states of aggregation | Salmonella enterica subsp. enterica serovar Typhimurium |
| additional information | - |
enzyme occurs in multiple states of aggregation | Homo sapiens |
| additional information | - |
enzyme occurs in multiple states of aggregation | Columba sp. |
| additional information | - |
the distribution among the states of aggregation is altered by a variety of conditions | Columba sp. |
| 34500 | - |
x * 34500, SDS-PAGE | Homo sapiens |
| 43000 | - |
x * 43000, sucrose density gradient sedimentation in the presence of urea 2 M combined with values of gel filtration | Homo sapiens |
| 53000 | - |
x * 53000, sucrose density gradient sedimentation in the presence of NaCl, 1 M, combined with values of gel filtration | Homo sapiens |
| 60000 | - |
x * 60000, sucrose density gradient sedimentation in phosphate buffer combined with values of gel filtration | Homo sapiens |
| 250000 | 600000 | sucrose density gradient sedimentation | Salmonella enterica subsp. enterica serovar Typhimurium |
| 700000 | 1200000 | - |
Rattus norvegicus |
| 700000 | 1200000 | sucrose density gradient sedimentation and gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose 5-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | Escherichia coli | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | Homo sapiens | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | Rattus norvegicus | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | Columba sp. | - |
AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Columba sp. | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Gallus gallus | - |
chicken | - |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
mouse | - |
| Ophiodon elongatus | - |
lingcod | - |
| Rattus norvegicus | - |
rat | - |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate and isoelectric precipitations, purified to near homogeneity | Salmonella enterica subsp. enterica serovar Typhimurium |
| to near homogeneity. Use of Mg2+, ATP and sulfhydryl compounds to stabilize the enzyme permit its isolation from erythrocytes | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | mechanism | Salmonella enterica subsp. enterica serovar Typhimurium | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | mechanism | Gallus gallus | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | mechanism | Mus musculus | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | mechanism | Escherichia coli | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | mechanism | Homo sapiens | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | mechanism | Rattus norvegicus | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | mechanism | Ophiodon elongatus | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | mechanism | Columba sp. | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi-bi reaction with ribose-5-phosphate binding first and 5-phosphoribose diphosphate being released last | Salmonella enterica subsp. enterica serovar Typhimurium | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi-bi reaction with ribose-5-phosphate binding first and 5-phosphoribose diphosphate being released last | Homo sapiens | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi-bi reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
| ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi-bi reaction | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Ehrlich ascites carcinoma cell | tumor cell line | Mus musculus | - |
| erythrocyte | - |
Homo sapiens | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Columba sp. | - |
| muscle | - |
Ophiodon elongatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 6-deoxyhomoribose 6-phosphonate | - |
Mus musculus | AMP + 6-deoxyhomoribose 6-phosphonate 1-diphosphate | - |
r | |
| ATP + 6-deoxyhomoribose 6-phosphonate | - |
Homo sapiens | AMP + 6-deoxyhomoribose 6-phosphonate 1-diphosphate | - |
r | |
| ATP + 6-deoxyhomoribose 6-phosphonate | - |
Rattus norvegicus | AMP + 6-deoxyhomoribose 6-phosphonate 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | - |
Escherichia coli | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | - |
Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | - |
Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | - |
Columba sp. | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphate | - |
Gallus gallus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphate | - |
Escherichia coli | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphate | - |
Ophiodon elongatus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphate | highly specific for ATP | Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as PRPP | r | |
| ATP + D-ribose 5-phosphate | highly specific for ATP | Columba sp. | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as PRPP | r | |
| ATP + D-ribose 5-phosphate | highly specific for ATP | Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphate | highly specific for ATP | Mus musculus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphate | highly specific for ATP | Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphate | highly specific for ATP | Columba sp. | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphate | highly specific for D-ribose 5-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as PRPP | r | |
| ATP + D-ribose 5-phosphate | highly specific for D-ribose 5-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphate | highly specific for D-ribose 5-phosphate | Homo sapiens | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphate | highly specific for D-ribose 5-phosphate | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphate | maximal velocity of the reverse reaction is 9-10% of the forward reaction | Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as PRPP | r | |
| ATP + D-ribose 5-phosphate | maximal velocity of the reverse reaction is 9-10% of the forward reaction | Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate | r | |
| ATP + D-ribose 5-phosphothionate | - |
Mus musculus | AMP + 5-phosphotionate-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphothionate | - |
Homo sapiens | AMP + 5-phosphotionate-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + D-ribose 5-phosphothionate | - |
Rattus norvegicus | AMP + 5-phosphotionate-alpha-D-ribose 1-diphosphate | - |
r | |
| CTP + D-ribose 5-phosphate | does not serve as substrate | Salmonella enterica subsp. enterica serovar Typhimurium | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| CTP + D-ribose 5-phosphate | does not serve as substrate | Mus musculus | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| CTP + D-ribose 5-phosphate | does not serve as substrate | Homo sapiens | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| CTP + D-ribose 5-phosphate | does not serve as substrate | Columba sp. | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| dATP + D-ribose 5-phosphate | - |
Rattus norvegicus | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| dATP + D-ribose 5-phosphate | in erythrocyte enzyme and tumor cell enzyme | Mus musculus | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| dATP + D-ribose 5-phosphate | in erythrocyte enzyme and tumor cell enzyme | Homo sapiens | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| additional information | - |
Rattus norvegicus | ? | - |
? | |
| additional information | 2-deoxyribose-5-phosphate is not substrate | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | D-ribitol-5-phosphate, alpha-methyl-D-ribofuranoside-5-phosphate, beta-methyl-D-ribofuranoside-5-phosphate are no substrates | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | other nucleosides di and triphosphates than ATP do not serve as substrates | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | other nucleosides di and triphosphates than ATP do not serve as substrates | Mus musculus | ? | - |
? | |
| additional information | other nucleosides di and triphosphates than ATP do not serve as substrates | Homo sapiens | ? | - |
? | |
| additional information | other nucleosides di and triphosphates than ATP do not serve as substrates | Columba sp. | ? | - |
? | |
| UTP + D-ribose 5-phosphate | does not serve as substrate | Salmonella enterica subsp. enterica serovar Typhimurium | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| UTP + D-ribose 5-phosphate | does not serve as substrate | Mus musculus | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| UTP + D-ribose 5-phosphate | does not serve as substrate | Homo sapiens | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
| UTP + D-ribose 5-phosphate | does not serve as substrate | Columba sp. | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Rattus norvegicus |
| ? | x * 32000-35000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| ? | x * 34500, SDS-PAGE | Homo sapiens |
| ? | x * 43000, sucrose density gradient sedimentation in the presence of urea 2 M combined with values of gel filtration | Homo sapiens |
| ? | x * 53000, sucrose density gradient sedimentation in the presence of NaCl, 1 M, combined with values of gel filtration | Homo sapiens |
| ? | x * 60000, sucrose density gradient sedimentation in phosphate buffer combined with values of gel filtration | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.4 | erythrocyte enzyme | Homo sapiens |
| 8 | 8.6 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
