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Literature summary for 2.7.6.1 extracted from

  • Ashihara, H.
    Characterization of phosphoribosylpyrophosphate synthetase from spinach leaves (1977), Z. Pflanzenphysiol., 83, 379-392.
No PubMed abstract available
Search for more literature for 2.7.6.1

Inhibitors

Inhibitors Comment Organism Structure
phosphate IC50 of 28 mM Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol more than 95% of the total activity Spinacia oleracea 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ in activation Spinacia oleracea
Mg2+ requirement Spinacia oleracea
Mn2+ can partially replace Mg2+ Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
 spinach
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, chromatography on DEAE-cellulose and ultrafiltration Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 10fold greater in cytosol than in the particulate fraction Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribose 5-phosphate
-
 Spinacia oleracea AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Spinacia oleracea

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
28
-
 IC50 of 28 mM Spinacia oleracea phosphate