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Literature summary for 2.7.4.B1 extracted from
- Characteristics, substrate analysis, and intracellular location of Saccharomyces cerevisiae UMP kinase (1993), Arch. Biochem. Biophys., 304, 197-204.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithio-bis(2-nitrobenzoic acid) | - |
Saccharomyces cerevisiae |  |
| N-ethylmaleimide | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | 80% | Saccharomyces cerevisiae | 5737 | - |
| nucleus | 20% | Saccharomyces cerevisiae | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 57% activity at 7.5 mM | Saccharomyces cerevisiae | |
| Cd2+ | no activity at 7.5 mM | Saccharomyces cerevisiae | |
| Co2+ | 99% activity at 7.5 mM | Saccharomyces cerevisiae | |
| Cu2+ | 11% activity at 7.5 mM | Saccharomyces cerevisiae | |
| Fe2+ | 24% activity at 7.5 mM | Saccharomyces cerevisiae | |
| Mg2+ | 100% activity at 7.5 mM | Saccharomyces cerevisiae | |
| Mn2+ | 100% activity at 7.5 mM | Saccharomyces cerevisiae | |
| Ni2+ | 84% activity at 7.5 mM | Saccharomyces cerevisiae | |
| Zn2+ | 14% activity at 7.5 mM | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
monomer, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UMP | Saccharomyces cerevisiae | - |
ADP + UDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P15700 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-iodo-dUMP | reaction occurs to a lesser extent | Saccharomyces cerevisiae | ADP + 5-iodo-dUDP | - |
? | |
| ATP + dGMP | reaction occurs to a lesser extent | Saccharomyces cerevisiae | ADP + dGDP | - |
? | |
| ATP + dTMP | reaction occurs to a lesser extent | Saccharomyces cerevisiae | ADP + dTDP | - |
? | |
| ATP + dUMP | - |
Saccharomyces cerevisiae | ADP + dUDP | - |
? | |
| ATP + GMP | reaction occurs to a lesser extent | Saccharomyces cerevisiae | ADP + GDP | - |
? | |
| ATP + IMP | reaction occurs to a lesser extent | Saccharomyces cerevisiae | ADP + IDP | - |
? | |
| ATP + UMP | - |
Saccharomyces cerevisiae | ADP + UDP | - |
? | |
| ATP + UMP | pH 7.5, 7.5 mM Mg2+, 30°C | Saccharomyces cerevisiae | ADP + UDP | - |
? | |
| ATP + XMP | reaction occurs to a lesser extent | Saccharomyces cerevisiae | ADP + XDP | - |
? | |
| CTP + UMP | reaction rate is 10-14% of that with ATP or dATP | Saccharomyces cerevisiae | CDP + UDP | - |
? | |
| dATP + UMP | - |
Saccharomyces cerevisiae | dADP + UDP | - |
? | |
| dCTP + UMP | reaction rate is 30-50% of that with ATP or dATP | Saccharomyces cerevisiae | dCDP + UDP | - |
? | |
| dGTP + UMP | reaction rate is 30-50% of that with ATP or dATP | Saccharomyces cerevisiae | dGDP + UDP | - |
? | |
| dTTP + UMP | reaction rate is 30-50% of that with ATP or dATP | Saccharomyces cerevisiae | dTDP + UDP | - |
? | |
| GTP + UMP | reaction rate is 30-50% of that with ATP or dATP | Saccharomyces cerevisiae | GDP + UDP | - |
? | |
| UTP + UMP | reaction rate is 10-14% of that with ATP or dATP | Saccharomyces cerevisiae | UDP + UDP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UMP kinase | - |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
enzyme activity decreases to 55%. In the presence of 1 mM UTP enzyme activity decreases to 73%. In the presence of 10% glycerol enzyme activity decreases to 77%. In the presence of 10% polyethylene glycol enzyme activity decreases to 95%. In the presence of 10 mM DTT enzyme activity decreases to 80% | Saccharomyces cerevisiae |
| 60 | - |
enzyme activity decreases to 3%. In the presence of 1 mM UTP enzyme activity decreases to 10%. In the presence of 10% glycerol enzyme activity decreases to 14%. In the presence of 10% polyethylene glycol enzyme activity decreases to 34%. In the presence of 10 mM DTT enzyme activity decreases to 68% | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | - |
Saccharomyces cerevisiae |
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