Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Wolbachia endosymbiont of Brugia malayi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-azido-3'-deoxythymidine | specific inhibitory effect on the enzyme activity, and these effect is in good association with binding interactions and the scoring functions | Wolbachia endosymbiont of Brugia malayi | |
5-bromo-2'-deoxyuridine | specific inhibitory effect on the enzyme activity, and these effect is in good association with binding interactions and the scoring functions | Wolbachia endosymbiont of Brugia malayi | |
5-chloro-2'-deoxyuridine | specific inhibitory effect on the enzyme activity, and these effect is in good association with binding interactions and the scoring functions | Wolbachia endosymbiont of Brugia malayi | |
albendazole | - |
Wolbachia endosymbiont of Brugia malayi | |
ATP | - |
Wolbachia endosymbiont of Brugia malayi | |
diethylcarbamazine | - |
Wolbachia endosymbiont of Brugia malayi | |
dTDP | competitive with dTMP | Wolbachia endosymbiont of Brugia malayi | |
ivermectin | - |
Wolbachia endosymbiont of Brugia malayi | |
levamisole | - |
Wolbachia endosymbiont of Brugia malayi | |
suramin | - |
Wolbachia endosymbiont of Brugia malayi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
dTMP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
0.066 | - |
ATP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | may substitute for Mg2+ | Wolbachia endosymbiont of Brugia malayi | |
Mg2+ | most favourable divalent cation | Wolbachia endosymbiont of Brugia malayi | |
Mn2+ | 52% of the activtiy with Mg2+ | Wolbachia endosymbiont of Brugia malayi | |
additional information | presence of a divalent cation is required. In presence of Ca2+, Cu2+, less than 10% of maximum activity, no activity with Zn2+ | Wolbachia endosymbiont of Brugia malayi | |
Ni2+ | 10% of the activity with Mg2+ | Wolbachia endosymbiont of Brugia malayi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
2 * 24000, SDS-PAGE | Wolbachia endosymbiont of Brugia malayi |
52000 | - |
gel filtration | Wolbachia endosymbiont of Brugia malayi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wolbachia endosymbiont of Brugia malayi | Q5GTD5 | - |
- |
Wolbachia endosymbiont of Brugia malayi TRS | Q5GTD5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dTMP | - |
Wolbachia endosymbiont of Brugia malayi | ADP + dTDP | - |
? | |
ATP + dTMP | - |
Wolbachia endosymbiont of Brugia malayi TRS | ADP + dTDP | - |
? | |
ATP + dUMP | - |
Wolbachia endosymbiont of Brugia malayi | ADP + dUDP | 12% of the activity with dTMP | ? | |
ATP + dUMP | - |
Wolbachia endosymbiont of Brugia malayi TRS | ADP + dUDP | 12% of the activity with dTMP | ? | |
dATP + dTMP | 26.9% of the activity with ATP | Wolbachia endosymbiont of Brugia malayi | dADP + dTDP | - |
? | |
dATP + dTMP | 26.9% of the activity with ATP | Wolbachia endosymbiont of Brugia malayi TRS | dADP + dTDP | - |
? | |
additional information | maximal activity is achieved at equimolar concentrations of ATP and Mg2+ | Wolbachia endosymbiont of Brugia malayi | ? | - |
? | |
additional information | maximal activity is achieved at equimolar concentrations of ATP and Mg2+ | Wolbachia endosymbiont of Brugia malayi TRS | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 24000, SDS-PAGE | Wolbachia endosymbiont of Brugia malayi |
Synonyms | Comment | Organism |
---|---|---|
TMK | - |
Wolbachia endosymbiont of Brugia malayi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Wolbachia endosymbiont of Brugia malayi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
30% residual activity | Wolbachia endosymbiont of Brugia malayi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable below | Wolbachia endosymbiont of Brugia malayi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.1 | - |
ATP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Wolbachia endosymbiont of Brugia malayi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | marked loss of activity above or below | Wolbachia endosymbiont of Brugia malayi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
5-chloro-2'-deoxyuridine | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
0.024 | - |
3'-azido-3'-deoxythymidine | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
0.042 | - |
5-bromo-2'-deoxyuridine | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
0.21 | - |
ATP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
0.23 | - |
dTDP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.018 | - |
pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | suramin | |
0.14 | - |
pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | albendazole | |
0.15 | - |
pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | ivermectin | |
0.75 | - |
pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | diethylcarbamazine | |
1.2 | - |
pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | levamisole |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
577 | - |
ATP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi | |
2240 | - |
dTMP | pH 7.4, temperature not specified in the publication | Wolbachia endosymbiont of Brugia malayi |