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Literature summary for 2.7.4.9 extracted from

  • Tourneux, L.; Bucurenci, N.; Lascu, I.; sakamoto, H.; Briand, G.; Gilles, A.M.
    Substitution of an alanine residue for glycine 146 in TMP kinase from Escherichia coli is responsible for bacterial hypersensitivity to bromodeoxyuridine (1998), J. Bacteriol., 180, 4291-4293.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.9

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme and G146A variant in Escherichia coli BL21(DE3)/pDIA17 Escherichia coli

Protein Variants

Protein Variants Comment Organism
G146A mutation is accompanied by a small but significant enhancement of the thermodynamic stability, midpoint denaturation temperature is 3°C higher than that of the wild-type enzyme, midpoint transition is 3.3 M urea, compared to 3.0 M for the wild-type enzyme Escherichia coli

General Stability

General Stability Organism
midpoint transition is 3.0 M urea for the wild-type enzyme and 3.3 M for the G146A mutant enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 TMP pH 7.4, 30°C, wild-type enzyme Escherichia coli
0.04
-
 ATP pH 7.4, 30°C, independent of cosubstrate Escherichia coli
0.08
-
 5-bromo-2'-deoxyuridine 5'-monophosphate pH 7.4, 30°C, wild-type enzyme Escherichia coli
0.25
-
 dTMP pH 7.4, 30°C, mutant enzyme G146A Escherichia coli
0.28
-
 5-bromo-2'-deoxyuridine 5'-monophosphate pH 7.4, 30°C, mutant enzyme G146A Escherichia coli
2.5
-
 dUMP pH 7.4, 30°C, wild-type enzyme Escherichia coli
4
-
 dUMP pH 7.4, 30°C, mutant enzyme G146A Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23779
-
 2 * 23779, electrospray ionization mass spectrometry, wild-type enzyme Escherichia coli
23794
-
 2 * 23794, electrospray ionization mass spectrometry, G146A mutant enzyme Escherichia coli
48000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-bromo-2'-deoxyuridine 5'-monophosphate
-
 Escherichia coli ADP + 5-bromo-2'-deoxyuridine 5'-diphosphate
-
 ?
ATP + dTMP
-
 Escherichia coli ADP + dTDP
-
 ?
ATP + dUMP
-
 Escherichia coli ADP + dUDP
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23779, electrospray ionization mass spectrometry, wild-type enzyme Escherichia coli
dimer 2 * 23794, electrospray ionization mass spectrometry, G146A mutant enzyme Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
 midpoint denaturation temperature for wild-type enzyme Escherichia coli
60
-
 midpoint denaturation temperature for the G146A mutant enzyme Escherichia coli