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Literature summary for 2.7.4.9 extracted from

  • Nelson, D.J.; Carter, C.E.
    Purification and characterization of Thymidine 5-monophosphate kinase from Escherichia coli B (1969), J. Biol. Chem., 244, 5254-5262.
    View publication on PubMed
Search for more literature for 2.7.4.9

Inhibitors

Inhibitors Comment Organism Structure
5-iodo-2'-dUMP
-
 Escherichia coli
dCTP
-
 Escherichia coli
dTTP
-
 Escherichia coli
dUMP
-
 Escherichia coli
NaCl 0.1 M, 25% inhibition Escherichia coli
NH4Cl 0.4 M, 75% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 dTMP pH 7.8, 37°C Escherichia coli
1.2
-
 ATP pH 7.8, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 18% of maximal activation with Mg2+ Escherichia coli
Mg2+ absolute requirement for divalent cation. When Mg2+ is equal to ATP, the rate of dTMP kinase reaction is maximal Escherichia coli
Mn2+ 41% of maximal activation with Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 gel filtration, sucrose density gradient centrifugation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, 1 year, 20-30% loss of activity Escherichia coli
2°C, half-life of about 1 month Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-iodo-2'-dUMP 60% of the maximal activity with dTMP Escherichia coli ADP + 5-iodo-2'-dUDP
-
 ?
ATP + dTMP
-
 Escherichia coli ADP + dTDP
-
 ?
ATP + dTMP
-
 Escherichia coli B / ATCC 11303 ADP + dTDP
-
 ?
ATP + dUMP at 15% of the activity with dTMP Escherichia coli ADP + dUDP
-
 ?
ATP + dUMP at 15% of the activity with dTMP Escherichia coli B / ATCC 11303 ADP + dUDP
-
 ?
CTP + dTMP about 30% of the activity with ATP Escherichia coli CDP + dTDP
-
 ?
CTP + dTMP about 30% of the activity with ATP Escherichia coli B / ATCC 11303 CDP + dTDP
-
 ?
dATP + dTMP about 80% of the activity with ATP Escherichia coli dADP + dTDP
-
 ?
dATP + dTMP about 80% of the activity with ATP Escherichia coli B / ATCC 11303 dADP + dTDP
-
 ?
dCTP + dTMP about 20% of the activity with ATP Escherichia coli dCDP + dTDP
-
 ?
dGTP + dTMP about 10% of maximal activity with ATP Escherichia coli dGDP + dTDP
-
 ?
dGTP + dTMP about 10% of maximal activity with ATP Escherichia coli B / ATCC 11303 dGDP + dTDP
-
 ?
dTTP + dTMP about 3% of the activity with ATP Escherichia coli dTDP
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 8.7 pH 7.0: about 50% of maximal activity, pH 8.7: about 40% of maximal activity Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
 5-iodo-2'-dUMP pH 7.8, 37°C Escherichia coli