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Literature summary for 2.7.4.8 extracted from

  • Kandeel, M.; Nakanishi, M.; Ando, T.; El-Shazly, K.; Yosef, T.; Ueno, Y.; Kitade, Y.
    Molecular cloning, expression, characterization and mutation of Plasmodium falciparum guanylate kinase (2008), Mol. Biochem. Parasitol., 159, 130-133.
    View publication on PubMed
Search for more literature for 2.7.4.8

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
D74A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Plasmodium falciparum
E101D site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Plasmodium falciparum
N103D site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Plasmodium falciparum
Y76F site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
AMP 10-15% inhibition at 5 mM Plasmodium falciparum
CMP 10-15% inhibition at 5 mM Plasmodium falciparum
dAMP 10-15% inhibition at 5 mM Plasmodium falciparum
dCMP 10-15% inhibition at 5 mM Plasmodium falciparum
dGMP competitive versus GMP and mixed-type versus ATP Plasmodium falciparum
EDTA 85% inhibition at 3 mM Plasmodium falciparum
additional information no inhibition by TMP and by 2-mercaptoethanol up to 5 mM Plasmodium falciparum
UMP 10-15% inhibition at 5 mM Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of wild-type and mutant enzymes Plasmodium falciparum
0.0222
-
 GMP wild-type enzyme Plasmodium falciparum
0.0592
-
 GMP mutant Y76F Plasmodium falciparum
0.0616
-
 GMP mutant E101D Plasmodium falciparum
0.0731
-
 dGMP mutant Y76F Plasmodium falciparum
0.0746
-
 dGMP wild-type enzyme Plasmodium falciparum
0.1816
-
 dGMP mutant D74A Plasmodium falciparum
0.1894
-
 dGMP mutant E101D Plasmodium falciparum
0.1933
-
 GMP mutant D74A Plasmodium falciparum
0.2265
-
 GMP mutant N103D Plasmodium falciparum
0.2364
-
 dGMP mutant N103D Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + GMP Plasmodium falciparum
-
 ADP + GDP
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
 with substrates ATP and dGMP Plasmodium falciparum
750
-
 with substrate ATP and GMP Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + AMP very low activity with AMP Plasmodium falciparum ADP + ADP
-
 ?
ATP + dGMP low activity, dGMP acts also as inhibitor Plasmodium falciparum ADP + dGDP
-
 ?
ATP + GMP
-
 Plasmodium falciparum ADP + GDP
-
 ?
additional information no activity with CMP, dTMP, dAMP, dCMP, and UMP Plasmodium falciparum ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 dGMP mutant Y76F Plasmodium falciparum
26.5
-
 dGMP mutant E101D Plasmodium falciparum
26.8
-
 dGMP mutant N103D Plasmodium falciparum
33.1
-
 dGMP mutant D74A Plasmodium falciparum
43
-
 dGMP wild-type enzyme Plasmodium falciparum
848
-
 GMP mutant E101D Plasmodium falciparum
946
-
 GMP wild-type enzyme Plasmodium falciparum
1032
-
 GMP mutant Y76F Plasmodium falciparum
1080
-
 GMP mutant N103D Plasmodium falciparum
1121
-
 GMP mutant D74A Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.8
-
 Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.148
-
 dGMP versus GMP Plasmodium falciparum
0.4
-
 dGMP versus ATP Plasmodium falciparum