Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged enzyme in COS cells, expression of His6-tagged and GST-tagged PSD-95 SH3-GK in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant PSD-95 SH3-GK which contains a deleterious point mutation in the C-terminal SH3 domain | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged PSD-95 SH3-GK from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant GST-tagged PSD-95 SH3-GK from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neuron | from mammalian | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | guanylate kinase binds a fragment of microtubule-associated protein-1a, i.e. MAP1a, in the GMP-binding site, the minimal GK binding site comprised by residues 1862-1878. MAP1a, which helps remodel the microtubule cytoskeleton in an activity-dependent manner, a is a common binding partner of PSD-95 GK and binds GK in an extended conformation, PSD-95 GK ligand consensus is not strongly constrained, binding via binding intermediate, structure, molecular dynamics simulation, overview | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structural model of microtubule-associated protein-1a. Domain organization of neuronal MAGUKs, overview | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
guanylate kinase | - |
Rattus norvegicus |
MAGUK PSD-95 | - |
Rattus norvegicus |
MAGUKs | - |
Rattus norvegicus |
membrane-associated guanylate kinases | - |
Rattus norvegicus |
postsynaptic density protein-95 SH3 GK | - |
Rattus norvegicus |
PSD-95 GK | - |
Rattus norvegicus |