Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Homo sapiens | |
5,5'-dithiobis(2-nitrobenzoate) | - |
Rattus norvegicus | |
additional information | no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol; no inhibition by guanosine, AMP, CMP, UMP, XMP, 6-thioinosine 5'-phosphate | Homo sapiens | |
additional information | no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol; no inhibition by guanosine, AMP, CMP, UMP, XMP, 6-thioinosine 5'-phosphate | Rattus norvegicus | |
additional information | no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol; no inhibition by guanosine, AMP, CMP, UMP, XMP, 6-thioinosine 5'-phosphate | Sus scrofa | |
N-ethylmaleimide | - |
Homo sapiens | |
N-ethylmaleimide | - |
Rattus norvegicus | |
p-chloromercuribenzoic acid | 1,4-dithiothreitol reverses | Homo sapiens | |
p-chloromercuribenzoic acid | 1,4-dithiothreitol reverses | Rattus norvegicus | |
p-chloromercuribenzoic acid | 1,4-dithiothreitol reverses | Sus scrofa | |
p-hydroxymercuribenzoate | - |
Homo sapiens | |
p-hydroxymercuribenzoate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
GMP | pH 7.5, 30ºC | Sus scrofa | |
0.01 | - |
GMP | pH 7.5, 30ºC | Rattus norvegicus | |
0.01 | - |
dGMP | pH 7.5, 30ºC | Rattus norvegicus | |
0.01 | - |
dGMP | pH 7.5, 30ºC | Sus scrofa | |
0.013 | - |
8-azaguanosine 5'-monophosphate | pH 7.5, 30ºC | Sus scrofa | |
0.018 | - |
GMP | pH 7.5, 30ºC | Homo sapiens | |
0.028 | - |
dGMP | pH 7.5, 30ºC | Rattus norvegicus | |
0.07 | - |
8-azaguanosine 5'-monophosphate | pH 7.5, 30ºC | Rattus norvegicus | |
0.074 | - |
dGMP | pH 7.5, 30ºC | Homo sapiens | |
0.091 | - |
8-azaguanosine 5'-monophosphate | pH 7.5, 30ºC | Homo sapiens | |
0.12 | - |
ATP | pH 7.5, 30ºC | Sus scrofa | |
0.18 | - |
ATP | pH 7.5, 30ºC | Rattus norvegicus | |
0.19 | - |
ATP | pH 7.5, 30ºC | Homo sapiens | |
2.1 | - |
6-thioguanosine 5'-monophosphate | pH 7.5, 30ºC | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no activator | Homo sapiens | |
Ca2+ | no activator | Rattus norvegicus | |
Ca2+ | no activator | Sus scrofa | |
K+ | activation | Homo sapiens | |
K+ | activation | Rattus norvegicus | |
K+ | activation | Sus scrofa | |
Li+ | partial activity | Sus scrofa | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Sus scrofa | |
Mn2+ | requirement | Homo sapiens | |
Mn2+ | requirement | Rattus norvegicus | |
Mn2+ | requirement | Sus scrofa | |
additional information | - |
Homo sapiens | |
additional information | - |
Rattus norvegicus | |
additional information | no activation by Sr2+ or Cs2+ | Sus scrofa | |
Na+ | activation | Sus scrofa | |
NH4+ | activation | Homo sapiens | |
NH4+ | activation | Rattus norvegicus | |
NH4+ | activation | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
method that includes DEAE-cellulose and Sephadex-75 chromatography | Rattus norvegicus |
method that includes DEAE-cellulose and Sephadex-75 chromatography | Sus scrofa |
method that includes DEAE-cellulose, Sephadex-75 chromatography and isoelectric focusing | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | - |
Sus scrofa | - |
erythrocyte | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
0°C, below, in 70% saturated ammonium sulfate, several years | Homo sapiens |
0°C, below, in 70% saturated ammonium sulfate, several years | Rattus norvegicus |
0°C, below, in 70% saturated ammonium sulfate, several years | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 6-thioguanosine 5'-monophosphate | - |
Homo sapiens | ADP + 8-thioguanosine 5'-diphosphate | - |
? | |
ATP + 6-thioguanosine 5'-monophosphate | - |
Rattus norvegicus | ADP + 8-thioguanosine 5'-diphosphate | - |
? | |
ATP + 6-thioguanosine 5'-monophosphate | - |
Sus scrofa | ADP + 8-thioguanosine 5'-diphosphate | - |
? | |
ATP + 8-azaguanosine 5'-monophosphate | - |
Homo sapiens | ADP + 8-azaguanosine 5'-diphosphate | - |
? | |
ATP + 8-azaguanosine 5'-monophosphate | - |
Rattus norvegicus | ADP + 8-azaguanosine 5'-diphosphate | - |
? | |
ATP + 8-azaguanosine 5'-monophosphate | - |
Sus scrofa | ADP + 8-azaguanosine 5'-diphosphate | - |
? | |
ATP + dGMP | - |
Homo sapiens | ADP + dGDP | - |
r | |
ATP + dGMP | - |
Rattus norvegicus | ADP + dGDP | - |
r | |
ATP + dGMP | - |
Sus scrofa | ADP + dGDP | - |
r | |
ATP + GMP | specificity | Homo sapiens | ADP + GDP | - |
? | |
ATP + GMP | specificity | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | specificity | Sus scrofa | ADP + GDP | - |
? | |
ATP + GMP | nucleoside monophosphate binding site is highly specific for guanine moiety | Homo sapiens | ADP + GDP | - |
? | |
ATP + GMP | nucleoside monophosphate binding site is highly specific for guanine moiety | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | nucleoside monophosphate binding site is highly specific for guanine moiety | Sus scrofa | ADP + GDP | - |
? | |
ATP + GMP | deoxyguanosine, guanosine are no acceptor substrates | Homo sapiens | ADP + GDP | - |
? | |
ATP + GMP | deoxyguanosine, guanosine are no acceptor substrates | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | deoxyguanosine, guanosine are no acceptor substrates | Sus scrofa | ADP + GDP | - |
? | |
ATP + GMP | 6-thio-IMP is no acceptor substrate | Homo sapiens | ADP + GDP | - |
? | |
ATP + GMP | 6-thio-IMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | 6-thio-IMP is no acceptor substrate | Sus scrofa | ADP + GDP | - |
? | |
ATP + GMP | XMP is no acceptor substrate | Homo sapiens | ADP + GDP | - |
? | |
ATP + GMP | XMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | XMP is no acceptor substrate | Sus scrofa | ADP + GDP | - |
? | |
ATP + GMP | AMP is no acceptor substrate | Homo sapiens | ADP + GDP | - |
? | |
ATP + GMP | AMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | AMP is no acceptor substrate | Sus scrofa | ADP + GDP | - |
? | |
ATP + GMP | CMP, UMP are no acceptor substrates | Homo sapiens | ADP + GDP | - |
? | |
ATP + GMP | CMP, UMP are no acceptor substrates | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | CMP, UMP are no acceptor substrates | Sus scrofa | ADP + GDP | - |
? | |
ATP + IMP | very poor substrate | Homo sapiens | ADP + IDP | - |
? | |
dATP + dGMP | - |
Homo sapiens | dADP + dGDP | - |
? | |
dATP + dGMP | - |
Rattus norvegicus | dADP + dGDP | - |
? | |
dATP + dGMP | - |
Sus scrofa | dADP + dGDP | - |
? | |
dATP + GMP | - |
Homo sapiens | dADP + GDP | - |
? | |
dATP + GMP | - |
Rattus norvegicus | dADP + GDP | - |
? | |
dATP + GMP | - |
Sus scrofa | dADP + GDP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
at least 15 min stable from pH 5.5-8.5 | Sus scrofa |
50 | - |
10 min, inactivation | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | at least 15 min stable at 30°C | Sus scrofa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | 3 isoelectric variants, predominant form: pI 4.9, agarose gel electrophoresis and isoelectric focusing | 4.8 | 4.7 |
Homo sapiens | 4 isoelectric variants, agarose gel electrophoresis and isoelectric focusing | - |
4.9 |