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Literature summary for 2.7.4.8 extracted from

  • Buccino, R.J.; Roth, J.S.
    Partial purification and properties of ATP:GMP phosphransferase from rat liver (1969), Arch. Biochem. Biophys., 132, 49-61.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by 1,4-dithiothreitol or 2-mercaptoethanol Rattus norvegicus

General Stability

General Stability Organism
2-mercaptoethanol does not stabilize Rattus norvegicus
freeze-thawing rapidly inactivates Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Rattus norvegicus
6-Thioguanosine 5'-phosphate dGMP-phosphorylation; not thiodeoxyguanosine derivative Rattus norvegicus
8-azaguanosine 5'-monophosphate (d)GMP-phosphorylation Rattus norvegicus
8-bromoguanosine 5'-monophosphate (d)GMP-phosphorylation Rattus norvegicus
Cs+ strong Rattus norvegicus
indol-3-acetic acid GMP + ATP protect Rattus norvegicus
Li+ strong Rattus norvegicus
additional information GMP-phosphorylation is less sensitive to metal ions than dGMP-phosphorylation; minimal inactivation by iodoacetate and iodoacetamide not affected by the presence or absence of KCl; no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol Rattus norvegicus
N-ethylmaleimide
-
Rattus norvegicus
p-hydroxymercuribenzoate
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Rattus norvegicus
K+ activation Rattus norvegicus
K+ dGMP, not GMP as substrate Rattus norvegicus
Mg2+ requirement Rattus norvegicus
Mg2+ active substrate: MgATP2- Rattus norvegicus
additional information
-
Rattus norvegicus
Na+ no activator Rattus norvegicus
NH4+ activation Rattus norvegicus
NH4+ less effective than K+ Rattus norvegicus
Rb+ activation, less effective than K+ Rattus norvegicus
Zn2+ activation Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20500
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
female Fischer or male Buffalo rats
-

Purification (Commentary)

Purification (Comment) Organism
partial by a method that includes DEAE-cellulose, hydroxyapatite and Sephadex G-200 chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hepatoma cell Morris 7793 or Dunning Rattus norvegicus
-
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-15°C, more than 6 months Rattus norvegicus
0-4°C, 6-10 weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 6-thioguanosine 5'-monophosphate not thiodeoxyguanosine derivative Rattus norvegicus ADP + 8-thioguanosine 5'-diphosphate
-
?
ATP + 8-azaguanosine 5'-monophosphate
-
Rattus norvegicus ADP + 8-azaguanosine 5'-diphosphate
-
?
ATP + 8-bromoguanosine 5'-monophosphate poor substrate Rattus norvegicus ADP + 8-bromoguanosine 5'-diphosphate
-
?
ATP + dGMP
-
Rattus norvegicus ADP + dGDP
-
r
ATP + GMP specificity Rattus norvegicus ADP + GDP
-
?
ATP + GMP deoxyguanosine, guanosine are no acceptor substrates Rattus norvegicus ADP + GDP
-
?
ATP + GMP TMP is not an acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP dCMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP IMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP XMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP AMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP dAMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
?
ATP + GMP CMP, UMP are no acceptor substrates Rattus norvegicus ADP + GDP
-
?
dATP + dGMP
-
Rattus norvegicus dADP + dGDP
-
?
dATP + GMP
-
Rattus norvegicus dADP + GDP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus