Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by 1,4-dithiothreitol or 2-mercaptoethanol | Rattus norvegicus |
General Stability | Organism |
---|---|
2-mercaptoethanol does not stabilize | Rattus norvegicus |
freeze-thawing rapidly inactivates | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Rattus norvegicus | |
6-Thioguanosine 5'-phosphate | dGMP-phosphorylation; not thiodeoxyguanosine derivative | Rattus norvegicus | |
8-azaguanosine 5'-monophosphate | (d)GMP-phosphorylation | Rattus norvegicus | |
8-bromoguanosine 5'-monophosphate | (d)GMP-phosphorylation | Rattus norvegicus | |
Cs+ | strong | Rattus norvegicus | |
indol-3-acetic acid | GMP + ATP protect | Rattus norvegicus | |
Li+ | strong | Rattus norvegicus | |
additional information | GMP-phosphorylation is less sensitive to metal ions than dGMP-phosphorylation; minimal inactivation by iodoacetate and iodoacetamide not affected by the presence or absence of KCl; no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol | Rattus norvegicus | |
N-ethylmaleimide | - |
Rattus norvegicus | |
p-hydroxymercuribenzoate | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Rattus norvegicus | |
K+ | activation | Rattus norvegicus | |
K+ | dGMP, not GMP as substrate | Rattus norvegicus | |
Mg2+ | requirement | Rattus norvegicus | |
Mg2+ | active substrate: MgATP2- | Rattus norvegicus | |
additional information | - |
Rattus norvegicus | |
Na+ | no activator | Rattus norvegicus | |
NH4+ | activation | Rattus norvegicus | |
NH4+ | less effective than K+ | Rattus norvegicus | |
Rb+ | activation, less effective than K+ | Rattus norvegicus | |
Zn2+ | activation | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20500 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
female Fischer or male Buffalo rats | - |
Purification (Comment) | Organism |
---|---|
partial by a method that includes DEAE-cellulose, hydroxyapatite and Sephadex G-200 chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatoma cell | Morris 7793 or Dunning | Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-15°C, more than 6 months | Rattus norvegicus |
0-4°C, 6-10 weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 6-thioguanosine 5'-monophosphate | not thiodeoxyguanosine derivative | Rattus norvegicus | ADP + 8-thioguanosine 5'-diphosphate | - |
? | |
ATP + 8-azaguanosine 5'-monophosphate | - |
Rattus norvegicus | ADP + 8-azaguanosine 5'-diphosphate | - |
? | |
ATP + 8-bromoguanosine 5'-monophosphate | poor substrate | Rattus norvegicus | ADP + 8-bromoguanosine 5'-diphosphate | - |
? | |
ATP + dGMP | - |
Rattus norvegicus | ADP + dGDP | - |
r | |
ATP + GMP | specificity | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | deoxyguanosine, guanosine are no acceptor substrates | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | TMP is not an acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | dCMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | IMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | XMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | AMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | dAMP is no acceptor substrate | Rattus norvegicus | ADP + GDP | - |
? | |
ATP + GMP | CMP, UMP are no acceptor substrates | Rattus norvegicus | ADP + GDP | - |
? | |
dATP + dGMP | - |
Rattus norvegicus | dADP + dGDP | - |
? | |
dATP + GMP | - |
Rattus norvegicus | dADP + GDP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |